Ting Y L, Sherr D, Degani H
Department of Chemical Physics, Weizmann Institute of Science, Rehovot, Israel.
Anticancer Res. 1996 May-Jun;16(3B):1381-8.
By comparing the metabolism of human mammary epithelial cells and human breast cancer cells (MCF7 and T47D), proliferating at approximately the same rate, it was possible to isolate the effect of malignancy alone on the energetics and phospholipid metabolism of cancer cells. 31p NMR of perfused mammary cells and of water soluble extracts of these cells showed that the levels of phosphocholine, phosphoethanolamine, and glycerol derivatives of these metabolites were very low and significantly less than that in the cancer cells, suggesting an association of malignancy with induction of phospholipid biosynthesis and breakdown. The level of the high energy phosphates and the rates of glucose consumption and aerobic glycolysis did not reveal distinct differences between normal and cancer cells. The comparable energetic appear to be related to the similarity of proliferating capacity in culture of the normal and cancer cells.
通过比较增殖速率大致相同的人乳腺上皮细胞和人乳腺癌细胞（MCF7和T47D）的代谢情况，能够单独分离出恶性肿瘤对癌细胞能量学和磷脂代谢的影响。对灌注乳腺细胞及其水溶性提取物进行的³¹P NMR分析表明，这些代谢物的磷酸胆碱、磷酸乙醇胺和甘油衍生物水平非常低，且显著低于癌细胞中的水平，这表明恶性肿瘤与磷脂生物合成和分解的诱导有关。高能磷酸盐水平以及葡萄糖消耗和有氧糖酵解速率在正常细胞和癌细胞之间并未显示出明显差异。这种相当的能量学似乎与正常细胞和癌细胞在培养中的增殖能力相似性有关。
