Institute of Neuroscience and Medicine (INM-3), Research Centre Jülich, 52425, Jülich, Germany,
Mol Imaging Biol. 2013 Oct;15(5):576-84. doi: 10.1007/s11307-013-0625-z.
The purpose of this study is to validate the feasibility of a voxel-based analysis of in vivo amyloid-β positron emission tomography (PET) imaging studies in transgenic mouse models of Alzheimer's disease.
We performed [(11)C]PiB PET imaging in 20 APP/PS1 mice and 16 age-matched controls, and histologically determined the individual amyloid-β plaque load. Using SPM software, we performed a voxel-based group comparison plus a regression analysis between PiB retention and actual plaque load, both thresholded at p FWE < 0.05. In addition, we carried out an individual ROI analysis in every animal.
The automated voxel-based group comparison allowed us to identify voxels with significantly increased PiB retention in the cortical and hippocampal regions in transgenic animals compared to controls. The voxel-based regression analysis revealed a significant association between this signal increase and the actual cerebral plaque load. The validity of these results was corroborated by the individual ROI-based analysis.
Voxel-based analysis of in vivo amyloid-β PET imaging studies in mouse models of Alzheimer's disease is feasible and allows studying the PiB retention patterns in whole brain maps. Furthermore, the selected approach in our study also allowed us to establish a quantitative relation between tracer retention and actual plaque pathology in the brain in a voxel-wise manner.
本研究旨在验证基于体素的阿尔茨海默病转基因小鼠模型体内淀粉样蛋白-β正电子发射断层扫描（PET）成像研究的可行性。
我们对 20 只 APP/PS1 小鼠和 16 只年龄匹配的对照小鼠进行了[(11)C]PiB PET 成像，并通过组织学确定了个体淀粉样蛋白-β斑块负荷。使用 SPM 软件，我们对 PiB 保留与实际斑块负荷进行了基于体素的组间比较和回归分析，两者均以 p FWE<0.05 为阈值。此外，我们对每只动物进行了个体 ROI 分析。
自动基于体素的组间比较使我们能够识别出转基因动物皮质和海马区 PiB 保留明显增加的体素。基于体素的回归分析显示，这种信号增加与大脑斑块负荷之间存在显著相关性。个体 ROI 分析进一步证实了这些结果的有效性。
基于体素的阿尔茨海默病转基因小鼠模型体内淀粉样蛋白-β PET 成像研究是可行的，可用于研究全脑图谱中的 PiB 保留模式。此外，我们研究中选择的方法还允许我们以体素为基础的方式建立示踪剂保留与大脑中实际斑块病理学之间的定量关系。
