天然抗菌剂和化学防腐剂对寄生曲霉生长的影响。
Effect of naturally occurring antimicrobials and chemical preservatives on the growth of Aspergillus Parasiticus.
机构信息
Food Protectants and Infestation Control Department, Central Food Technological Research Institute (CSIR), Mysore, 570 020 India.
出版信息
J Food Sci Technol. 2012 Apr;49(2):228-33. doi: 10.1007/s13197-011-0275-6. Epub 2011 Feb 12.
Effect of water activity (aw, 0.99), pH (4.5) and their interaction on the growth inhibition of Aspergillus parasiticus was studied on potato dextrose agar (PDA) using various antimicrobial agents (citral, carvacrol, eugenol, cineole, thymol guaiacol, vanillin, anethol, potassium sorbate and sorbic acid). The results demonstrate that colony diameter (mm) exhibited a constant increase with time (zero order kinetics) for all antimicrobials evaluated. Eugenol and sorbic acid inhibited the test fungi at 300 and 600 ppm, respectively. Radial growth rate (RGR) of A. parasiticus was significantly (p < 0.05) different among different antimicrobials as well as the concentrations tested. However, this difference was not observed with higher concentration of citral, eugenol, vanillin and sorbic acid. Among the antimicrobials evaluated potassium sorbate, cineole, anethol and guaiacol were least effective. Thymol, eugenol and carvacrol were more effective in inhibiting A. parasiticus even with low concentration (150 ppm) as their mean RGR was zero even after 20 days of incubation (pH 4.5).
水活度（aw，0.99）、pH（4.5）及其相互作用对寄生曲霉生长抑制的影响，采用不同的抗菌剂（柠檬醛、香芹酚、丁子香酚、桉油醇、麝香草酚、愈创木酚、茴香脑、山梨酸钾和山梨酸）在马铃薯葡萄糖琼脂（PDA）上进行了研究。结果表明，所有评估的抗菌剂的菌落直径（mm）均随时间呈恒定增加（零级动力学）。丁子香酚和山梨酸分别在 300 和 600 ppm 时抑制了测试真菌。寄生曲霉的径向生长速率（RGR）在不同的抗菌剂以及测试的浓度之间有显著差异（p<0.05）。然而，在较高浓度的柠檬醛、丁子香酚、愈创木酚和山梨酸中没有观察到这种差异。在所评估的抗菌剂中，山梨酸钾、桉油醇、茴香脑和麝香草酚的效果最差。百里香酚、丁子香酚和香芹酚即使在低浓度（150 ppm）时也更有效地抑制寄生曲霉，因为它们的平均 RGR 为零，即使在 20 天的孵育后（pH 4.5）也是如此。
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