Innovative production of fungal pulp from and its application in a fungal paper box containing clove oil.

The aims of this study were to develop fungal pulp from (white-rot fungi) and apply it with clove oil in a paper box to inhibit mold growth on the surface of peanuts. Broken rice media with different sugar solutions (2-10% w w) were prepared and then inoculated with mycelium at amounts from 0.5 to 1.5% w w. Fungal pulp and commercial paper (50 g) at different ratios (100:0, 70:30, 50:50, 30:70 and 0:100) were mixed and prepared before being placed into a stainless box (5 cm long by 5 cm wide). For the antimicrobial activity against on peanuts, a paper box was incorporated with 2.5, 5 and 7.5% w w of clove oil, eugenol, caryophyllene, and a combination of eugenol and caryophyllene at ratios of 7:1, 4:4, and 1:7. Results indicated that the highest fungal pulp biomass of in broken rice media was found when using 6% sugar with 1% mycelium inoculums. Fungal pulp and commercial paper at the ratio of 70:30 produced the highest value of hardness. The paper box containing clove oil at 7.5% w w inhibited on peanuts for at least 28 days while the control had mold growth within 3 days. Combining eugenol and caryophyllene, the main components of clove oil, at the ratio of 7:1 (7.5% w w) in the paper box should be a key factor to inhibit during storage.