应用 RAPD 和 ISSR 标记分析评估组培繁殖的枣椰树（Phoenix dactylifera L.）植株的遗传保真度。
Assessment of genetic fidelity of micropropagated date palm (Phoenix dactylifera L.) plants by RAPD and ISSR markers assay.
机构信息
Department of Agricultural Biotechnology, Anand Agricultural University, Anand, 388 110 Gujarat India.
出版信息
Physiol Mol Biol Plants. 2010 Apr;16(2):207-13. doi: 10.1007/s12298-010-0023-9. Epub 2010 Sep 5.
Abstract
RAPD (Random Amplified Polymorphic DNA) and ISSR (Inter-Simple Sequence Repeats) markers assay were employed to validate the genetic stability of date palm (Phoenix dactylifera L.) plants multiplied through somatic embryogenesis with upto forty two in vitro subcultures. Out of the 160 RAPD and 21 ISSR primers screened, 30 RAPD and 12 ISSR primers produced a total of 347 (246 RAPDs + 101 ISSRs) clear, distinct and reproducible amplicons, which were monomorphic across all micropropagated plants (27) studied. Thus, a total 8592 bands (number of plants analysed x number of amplicons with all the primers) were generated which exhibited homogeneous banding patterns with both RAPD and ISSR markers. These results indicate that the micropropagation protocol developed by us for rapid in vitro multiplication is appropriate and suitable for clonal propagation of date palm and corroborated the fact that somatic embryogenesis can also be used as one of the safest modes for production of true-to-type plants.
摘要
RAPD（随机扩增多态性 DNA）和 ISSR（简单重复序列间扩增）标记分析被用来验证通过体细胞胚胎发生繁殖的 42 次以上体外继代培养的枣（Phoenix dactylifera L.）植物的遗传稳定性。在筛选的 160 个 RAPD 和 21 个 ISSR 引物中，30 个 RAPD 和 12 个 ISSR 引物共产生了 347 个（246 个 RAPD + 101 个 ISSR）清晰、独特和可重复的扩增子，这些扩增子在所有研究的微繁殖植物（27 个）中都是单态的。因此，总共生成了 8592 个条带（分析的植物数量 x 所有引物的扩增子数量），这些条带与 RAPD 和 ISSR 标记均表现出均匀的带型。这些结果表明，我们为快速体外繁殖开发的微繁殖方案是合适的，适合枣的无性繁殖，并证实了体细胞胚胎发生也可以作为产生典型植物的最安全模式之一。
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6
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7
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