植物组织培养的遗传不稳定性：正常控制的崩溃
Genetic instability of plant tissue cultures: breakdown of normal controls.
作者信息
Phillips R L, Kaeppler S M, Olhoft P
机构信息
Department of Agronomy, University of Minnesota, St. Paul 55108.
出版信息
Proc Natl Acad Sci U S A. 1994 Jun 7;91(12):5222-6. doi: 10.1073/pnas.91.12.5222.
Abstract
Plants regenerated from relatively undifferentiated callus cultures possess a vast array of genetic changes. Such variations can result in useful agricultural and horticultural products. For other purposes, however, variations in traits other than those of interest may be undesirable--for example, using cultured cells for genetic engineering. Any steps made toward understanding the basis of tissue culture-induced genetic variation should be helpful in developing a more stable and manipulatable somatic cell system. This review provides a glimpse at the specific kinds of genetic changes encountered among regenerated plants and their progeny. Included among these variations are cytosine methylation alterations of the genome. The repeat-induced point mutation (RIP) phenomenon, reported for filamentous fungi, is invoked to provide a framework to consider the origin of variation in plant tissue cultures.
摘要
从相对未分化的愈伤组织培养物再生的植物具有大量的遗传变化。这种变异可产生有用的农业和园艺产品。然而，对于其他目的而言，除了感兴趣的性状之外的其他性状变异可能是不理想的——例如，将培养细胞用于基因工程。朝着理解组织培养诱导的遗传变异基础所采取的任何步骤都应有助于开发更稳定且可操控的体细胞系统。本综述简要介绍了再生植物及其后代中遇到的特定类型的遗传变化。这些变异包括基因组的胞嘧啶甲基化改变。丝状真菌中报道的重复诱导点突变（RIP）现象被用来提供一个框架，以考虑植物组织培养中变异的起源。
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