R&D Department, P.O. No. 28, KDHP Co. Pvt. Ltd., Munnar, Kerala India.
Physiol Mol Biol Plants. 2010 Dec;16(4):359-67. doi: 10.1007/s12298-010-0040-8. Epub 2011 Jan 9.
Various clones of tea [Camellia sinensis (L.) O. Kuntze] such as TTL-1, TTL-2, TTL-4, TTL-5, TTL-6, UPASI-2 and UPASI-3 planted in the field were subjected to soil moisture stress conditions by withholding irrigation. A control set of the same clones were maintained by watering regularly. The soil water content of the irrigated and non irrigated plants was monitored through the soil moisture status. The extent of effect of drought on tea plants were monitored through various physiological parameters such as shoot weight, leaf water potential, chlorophyll and carotenoid content, chlorophyll fluorescence (Fv/Fm), net photosynthetic rate, transpiration rate, stomatal conductance and biochemical parameters such as extent of proline accumulation and free radical generation. These parameters were studied on the 30 d of non irrigation and on the 5 d during recovery from drought. The plants recovered when re-irrigated after 30 d of non-irrigation, which suggests that permanent wilting did not occur due to non-irrigation up to 30 d. On the 30 d of non-irrigation the clones TTL-1, TTL-6 and UPASI-2 showed lesser reduction of shoot weight, leaf water potential, chlorophyll fluorescence, photosynthetic rate, transpiration rate and stomatal conductance and increased proline and lesser lipid peroxidation as compared to the other clones. From these results it can be concluded that the clones TTL-1, TTL-6 and UPASI-2 are comparatively more drought tolerant than the clones TTL-2, TTL-4, TTL-5 and UPASI-3.
各种茶树[Camellia sinensis (L.) O. Kuntze]克隆体,如 TTL-1、TTL-2、TTL-4、TTL-5、TTL-6、UPASI-2 和 UPASI-3,在田间种植后,通过断水来承受土壤水分胁迫条件。同一克隆体的对照组通过定期浇水来维持。通过土壤水分状况监测灌溉和未灌溉植物的土壤含水量。通过各种生理参数(如枝条重量、叶片水势、叶绿素和类胡萝卜素含量、叶绿素荧光(Fv/Fm)、净光合速率、蒸腾速率、气孔导度和生化参数(如脯氨酸积累和自由基生成程度)监测干旱对茶树的影响程度。这些参数在非灌溉的第 30 天和干旱恢复的第 5 天进行研究。在 30 天的非灌溉后重新灌溉时,植物恢复了生长,这表明由于非灌溉,植物没有永久性萎蔫,非灌溉长达 30 天。在第 30 天的非灌溉期,TTL-1、TTL-6 和 UPASI-2 克隆体的枝条重量、叶片水势、叶绿素荧光、光合速率、蒸腾速率和气孔导度的减少幅度较小,脯氨酸增加,脂质过氧化程度较低,与其他克隆体相比。从这些结果可以得出结论,TTL-1、TTL-6 和 UPASI-2 克隆体比 TTL-2、TTL-4、TTL-5 和 UPASI-3 克隆体更具耐旱性。