Department of Emergency Medicine, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China.
Evid Based Complement Alternat Med. 2013;2013:464650. doi: 10.1155/2013/464650. Epub 2013 Mar 13.
Shen-Fu injection (SFI) derived from the ancient traditional Chinese medicine. In this study, the effects of SFI on the expression of T-bet/GATA-3 and its potential mechanisms causing the shift of T cells from Th2 to Th1 on postresuscitation lung injury were examined in a porcine model of cardiac arrest. 30 pigs were randomly divided into SHAM (n = 6) and three return of spontaneous circulation (ROSC) groups (n = 8 per group); 24 pigs were subjected to 8 min of electrically induced cardiac arrest and 2 min of basic life support, which received central venous injection of Shen-Fu (SFI), epinephrine (EP) or saline (SA). After successful ROSC, 18 surviving pigs were sacrificed at 24 h after ROSC (n = 6 per group). The levels of serum and lung tissue interleukin (IL)-4 and interferon (IFN)- γ were measured by ELISA, and the protein and mRNA levels of GATA-3 and T-bet in the lung tissue were determined by western blotting and quantitative real-time polymerase chain reaction, respectively. Compared with the EP and SA groups, SFI treatment reduced the levels of IL-4 (P < 0.05), increased levels of IFN- γ (P < 0.05), and induced T-bet mRNA upregulation and GATA-3 mRNA downregulation (P < 0.05). SFI attenuated lung injury and regulated lung immune disorders. Therefore, SFI could protect postresuscitation lung injury by modulating a Th1/Th2 imbalance.
参附注射液源自古老的传统中药。在这项研究中，在猪心肺复苏后肺损伤模型中，研究了参附注射液对 T-bet/GATA-3 表达的影响及其导致 T 细胞从 Th2 向 Th1 转变的潜在机制。30 头猪随机分为 SHAM（n = 6）和三个自主循环恢复（ROSC）组（每组 n = 8）；24 头猪接受 8 分钟的电诱导心脏骤停和 2 分钟的基础生命支持，给予参附注射液（SFI）、肾上腺素（EP）或生理盐水（SA）中心静脉注射。ROSC 成功后，18 头幸存猪在 ROSC 后 24 小时（每组 n = 6）处死。通过 ELISA 测量血清和肺组织白细胞介素（IL）-4 和干扰素（IFN）-γ水平，通过 Western 印迹和定量实时聚合酶链反应分别测定肺组织中 GATA-3 和 T-bet 的蛋白和 mRNA 水平。与 EP 和 SA 组相比，SFI 治疗降低了 IL-4 水平（P < 0.05），增加了 IFN-γ 水平（P < 0.05），并诱导 T-bet mRNA 上调和 GATA-3 mRNA 下调（P < 0.05）。SFI 减轻了肺损伤并调节了肺部免疫紊乱。因此，SFI 通过调节 Th1/Th2 失衡来保护心肺复苏后肺损伤。
