Peterson C, Cotman C W
Department of Psychobiology, University of California, Irvine 92717.
Brain Res. 1990 May 7;515(1-2):39-44. doi: 10.1016/0006-8993(90)90573-t.
Medium conditioned by fibroblasts promotes survival and neurite outgrowth of cultured hippocampal neurons. Sub-confluent cultures of cells from normal young, aged and Alzheimer donors conditioned serum-free medium for 2 days. Hippocampal neurons from 18- to 19-day-old embryos were maintained in medium conditioned by skin fibroblasts from young, aged or Alzheimer donors. One, 4 and 7 days after plating the cells were examined for morphological differences. Neuron survival four days after plating was found to be the highest in medium conditioned by young greater than aged greater than Alzheimer cells. These findings suggest that both aged and Alzheimer donors secrete less of the substances that are necessary for survival and neurite outgrowth of hippocampal neurons. This provides further evidence that non-neuronal cells demonstrate abnormalities during aging and Alzheimer's disease.
成纤维细胞条件培养基可促进培养的海马神经元的存活和神经突生长。来自正常年轻、老年和阿尔茨海默病供体的亚汇合细胞培养物在无血清培养基中条件培养2天。将18至19日龄胚胎的海马神经元培养在由年轻、老年或阿尔茨海默病供体的皮肤成纤维细胞条件培养基中。接种后1天、4天和7天检查细胞的形态差异。发现接种后4天神经元在由年轻细胞条件培养基中的存活率最高，其次是老年细胞条件培养基，阿尔茨海默病细胞条件培养基中最低。这些发现表明，老年和阿尔茨海默病供体分泌的海马神经元存活和神经突生长所需物质较少。这进一步证明了非神经元细胞在衰老和阿尔茨海默病期间表现出异常。
