Uchida Y, Tomonaga M
Department of Clinical Pathology, Tokyo Metropolitan Institute of Gerontology, Japan.
Brain Res. 1989 Feb 27;481(1):190-3. doi: 10.1016/0006-8993(89)90503-9.
Cell cultures of neonatal rat cerebral cortex in a serum-free medium were used to investigate whether specific neurotrophic factors are accumulated or inhibitory factors for neuronal survival and neurite formation decrease in AD brain extract. Neurotrophic activity in brain extract was quantified by using the ELISA for microtubule-associated protein 2 (MAP2). Inhibitory factors, which blocked neurotrophic activity, were present in normal brain extract. In AD brain extract, loss of the inhibitory factors resulted in a relative increase in neurotrophic activity. The inhibitory factors in normal brain extract were retained on the ultrafiltration membrane with molecular weight cutoff of 10 kDa.
在无血清培养基中培养新生大鼠大脑皮层细胞，以研究在阿尔茨海默病（AD）脑提取物中是否存在特定的神经营养因子积累或神经元存活及神经突形成的抑制因子减少的情况。通过使用针对微管相关蛋白2（MAP2）的酶联免疫吸附测定（ELISA）来量化脑提取物中的神经营养活性。正常脑提取物中存在阻断神经营养活性的抑制因子。在AD脑提取物中，抑制因子的缺失导致神经营养活性相对增加。正常脑提取物中的抑制因子保留在截留分子量为10 kDa的超滤膜上。
