一种新型高分辨率熔解 PCR 检测法可区分嗜吞噬细胞无形体和“候选新立克次体”。
A novel high-resolution melt PCR assay discriminates Anaplasma phagocytophilum and "Candidatus Neoehrlichia mikurensis".
机构信息
Institute for Parasitology and Tropical Veterinary Medicine, Freie Universität Berlin, Berlin, Germany.
出版信息
J Clin Microbiol. 2013 Jun;51(6):1958-61. doi: 10.1128/JCM.00284-13. Epub 2013 Apr 10.
Abstract
"Candidatus Neoehrlichia mikurensis" (Anaplasmataceae) is an emerging pathogen transmitted by Ixodes ticks. Conventional PCR and the newly developed high-resolution melt PCR were used to detect and discriminate "Candidatus Neoehrlichia mikurensis" and Anaplasma phagocytophilum. Both bacterial species were frequently found in Ixodes ricinus and Ixodes hexagonus but virtually absent from Dermacentor reticulatus. In rodents, "Candidatus N. mikurensis" was significantly more prevalent than A. phagocytophilum, whereas in cats, only A. phagocytophilum was found.
摘要
"候选新立克次体（Neoehrlichia mikurensis）"（立克次体科）是一种由硬蜱传播的新兴病原体。常规 PCR 和新开发的高分辨率熔解曲线 PCR 用于检测和区分"候选新立克次体（Neoehrlichia mikurensis）"和嗜吞噬细胞无形体（Anaplasma phagocytophilum）。这两种细菌在硬蜱和璃眼蜱中经常发现，但在扇头蜱中几乎不存在。在啮齿动物中，"候选新立克次体（Neoehrlichia mikurensis）"的流行率明显高于嗜吞噬细胞无形体（Anaplasma phagocytophilum），而在猫中，只发现了嗜吞噬细胞无形体（Anaplasma phagocytophilum）。
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