Esquinas Paula, Botero Lucía, Patiño María Del Pilar, Gallego Carolina, Iregui Carlos
Departamento de Patología, Hospital Universitario Fundación Santafé de Bogotá, Bogotá, Colombia.
Vet Med Int. 2013;2013:321390. doi: 10.1155/2013/321390. Epub 2013 Mar 14.
An ultrastructural comparison between the nasal cavities of healthy rabbits and those suffering from two forms of spontaneous infection with Pasteurella multocida was undertaken. Twelve commercially produced rabbits of different ages and respiratory health status were divided into four groups: healthy from 0 to 21 days (G1, n = 2); healthy from 23 to 49 days (G2, n = 2); healthy from 51 to 69 days (G3, n = 2); diseased rabbits with septicemia and the rhinitic form of P. multocida infection (G4, n = 3). The main ultrastructural changes observed were a widening of the interepithelial spaces, increased activity and number of goblet cells, the formation of two types of vacuoles in epithelial cells, the degranulation and migration of heterophils between the epithelial cells, and the association of this migration with some of the other changes. No bacteria were observed adhering to the epithelium, and very few were observed free in the mucus. Scant inter-epithelial spaces were found in healthy rabbits, but they were not as large and numerous as those found in diseased animals. We discuss the origin and meaning of these changes but, we focus on the significance of the inter-epithelial spaces and goblet cells for the defense of the upper respiratory airways against the bacterium and its lipopolysaccharide.
对健康兔子的鼻腔与感染多杀性巴氏杆菌两种自发感染形式的兔子的鼻腔进行了超微结构比较。将12只不同年龄和呼吸健康状况的商业生产兔子分为四组：0至21天的健康兔子（G1，n = 2）；23至49天的健康兔子（G2，n = 2）；51至69天的健康兔子（G3，n = 2）；患有败血症和多杀性巴氏杆菌感染鼻炎形式的患病兔子（G4，n = 3）。观察到的主要超微结构变化包括上皮间隙增宽、杯状细胞活性和数量增加、上皮细胞中形成两种类型的液泡、异嗜性粒细胞在上皮细胞间脱颗粒和迁移，以及这种迁移与其他一些变化的关联。未观察到细菌附着在上皮细胞上，在黏液中游离的细菌也很少。在健康兔子中发现的上皮间隙很少，但不如患病动物中发现的那么大且多。我们讨论了这些变化的起源和意义，但重点关注上皮间隙和杯状细胞对上呼吸道抵御该细菌及其脂多糖的防御意义。
