Bonilla-Ruz L F, García-Delgado G A
Sección de Microbiología y Parasitología, Centro Interdisciplinario de Ciencias de la Salud, Instituto Politécnico Nacional, Edo. de México, Mexico.
Rev Latinoam Microbiol. 1993 Oct-Dec;35(4):361-9.
Adult clinically healthy New Zealand rabbits were sampled bacteriologically to detect carriers and non-carriers of Pasteurella multocida. Both groups of rabbits were killed separately to obtain samples of nasal, buccal, pharyngeal and tracheal epithelial cells. The cells were tested for adherence in vitro to 18 isolates of P. multocida from healthy and sick rabbits, from ovine, bovine, cat and swine. The number of bacteria adhered per cell up to 25 cells per preparation were registered. Analysis of variance was used to interpret the significance of results. Adherence of P. multocida was significantly higher to carrier rabbit cells than to non-carrier rabbit cells. Bacterial isolates from rabbits were more adherent to rabbit cells than to isolates from other species. The frequency was higher to buccal and pharyngeal cells than to nasal and tracheal cells. Isolates from healthy animals adhered better to rabbit cells than isolates from sick animals, except the isolates from sick animals which adhered better to nasal cells of non carriers than did isolates from healthy rabbits.
对成年临床健康的新西兰兔进行细菌学采样，以检测多杀性巴氏杆菌的携带者和非携带者。两组兔子分别处死，获取鼻、颊、咽和气管上皮细胞样本。检测这些细胞在体外对来自健康和患病兔子、绵羊、牛、猫和猪的18株多杀性巴氏杆菌分离株的黏附情况。记录每份制剂中每个细胞黏附的细菌数量，最多记录25个细胞。采用方差分析来解释结果的显著性。多杀性巴氏杆菌对携带菌兔细胞的黏附显著高于对非携带菌兔细胞的黏附。来自兔子的细菌分离株对兔细胞的黏附性比对来自其他物种的分离株更强。对颊细胞和咽细胞的黏附频率高于对鼻细胞和气管细胞的黏附频率。来自健康动物的分离株比对患病动物的分离株对兔细胞的黏附性更好，不过患病动物的分离株对非携带者的鼻细胞的黏附性比对健康兔子的分离株更好。
