Inoue T, Nakaoka Y
Department of Biophysical Engineering, Faculty of Engineering Science, Osaka University, Japan.
Cell Struct Funct. 1990 Apr;15(2):107-12. doi: 10.1247/csf.15.107.
A transient depolarization was recorded in response to the cooling of a deciliated Paramecium. The amplitude of the depolarization was almost proportional to the cooling rate. Therefore, the cells are sensitive to the rate of temperature change. The input resistance of the membrane transiently increased during the cooling. When constant current was applied to shift the resting membrane potential to a negative or positive level, the initial depolarization in response to cooling decreased, and the following hyperpolarization during cooling reversed to a gradual depolarization during a positive shift. The potential at which the reversal occurred was independent of K+ concentration and was slightly dependent on Ca2+ concentration (10 mV/log[Ca2+]o). The amplitude of the initial depolarization decreased with the increase in K+ and was not affected by Ca2+. These results are discussed in terms of changes in membrane conductances in response to cooling.
在对去纤毛草履虫进行冷却时记录到了短暂的去极化现象。去极化的幅度几乎与冷却速率成正比。因此，细胞对温度变化速率敏感。在冷却过程中，膜的输入电阻短暂增加。当施加恒定电流将静息膜电位向负向或正向移动时，冷却时的初始去极化减小，并且在正向移动期间冷却时随后的超极化转变为逐渐去极化。发生反转的电位与K⁺浓度无关，并且略微依赖于Ca²⁺浓度（10 mV/log[Ca²⁺]ₒ）。初始去极化的幅度随K⁺增加而减小，并且不受Ca²⁺影响。根据冷却时膜电导的变化对这些结果进行了讨论。
