Zhang D R, Yeh H H
Department of Neurobiology and Anatomy, University of Rochester Medical Center, NY 14642.
Brain Res Dev Brain Res. 1990 May 1;53(2):194-9. doi: 10.1016/0165-3806(90)90006-k.
The histogenesis of amacrine cells expressing corticotropin releasing factor-like immunoreactivity (CRF-LI) was examined in the rat retina by incorporating [3H]thymidine autoradiography and immunohistochemistry. Our results indicate that, during the period of amacrine cell generation, the population of CRF-LI amacrine cells are generated within a window of time from gestational days (ED)-16 to -20; the majority of them are produced by ED-18. No CRF-LI cells have been found to be 'born' postnatally. The histogenetic pattern of these cells follows a center-to-periphery gradient. Furthermore, CRF-LI cells in both the inner nuclear layer and the ganglion cell layer are generated in the same histogenetic wave and follow an identical temporo-spatial pattern. These results are consistent with and extend our previous findings of a differential change in CRF-LI cell density and total cell number during postnatal development.
通过结合[3H]胸腺嘧啶核苷放射自显影术和免疫组织化学方法，在大鼠视网膜中研究了表达促肾上腺皮质激素释放因子样免疫反应性（CRF-LI）的无长突细胞的组织发生。我们的结果表明，在无长突细胞产生期间，CRF-LI无长突细胞群体在妊娠天数（ED）-16至-20的时间窗口内产生；其中大多数在ED-18产生。未发现有CRF-LI细胞在出生后“出生”。这些细胞的组织发生模式遵循从中心到周边的梯度。此外，内核层和神经节细胞层中的CRF-LI细胞在同一组织发生波中产生，并遵循相同的时空模式。这些结果与我们之前关于出生后发育期间CRF-LI细胞密度和总细胞数差异变化的发现一致，并扩展了这些发现。
