The Mina & Everard Goodman Faculty of Life Sciences & Institute of Nanotechnology, Bar-Ilan University, Ramat Gan, Israel.
Nucleus. 2010 Nov-Dec;1(6):492-8. doi: 10.4161/nucl.1.6.13515. Epub 2010 Sep 3.
The transcription machinery in the eukaryotic nucleus generates messenger RNA molecules that translocate through the nucleoplasm, anchor to a nuclear pore, and find their way out into the cytoplasm. The dynamic aspects of these steps in the expression pathway were examined in order to understand the kinetic time-frames of gene activation and message dissemination. Utilizing live-cell imaging and tracking of single mRNPs containing different sized mRNAs and varying numbers of introns and exons, it was possible to quantify the temporal and spatial characteristics of the nucleoplasmic travels of mRNPs as well as the kinetics of translocation through the nuclear pore.
真核细胞核中的转录机制生成信使 RNA 分子，这些分子穿过核质，锚定在核孔上，并找到进入细胞质的途径。为了了解基因激活和信息传递的动力学时间框架，研究了表达途径中这些步骤的动态方面。利用活细胞成像和跟踪含有不同大小的 mRNA 以及不同数量的内含子和外显子的单个 mRNP，可以定量测量 mRNP 在核质中的时空特征以及穿过核孔的易位动力学。
