Institute for Theoretical Biology, Charité Universitätsmedizin Berlin, Invalidenstraße 43, 10115 Berlin, Germany.
Cell Rep. 2013 Apr 25;3(4):1228-38. doi: 10.1016/j.celrep.2013.03.013. Epub 2013 Apr 11.
In addition to the well-known circadian rhythms in animal behavior, cell biology, and gene expression, there are also 12 hr rhythms. The mechanism giving rise to these 12 hr rhythms is not clear. We worked with the hypothesis that observed 12 hr rhythms in gene expression are the results of an interplay between components of the circadian clock. Analysis of mouse liver expression data indeed revealed a strong circadian component in observed 12 hr gene expression rhythms. We show theoretically that pairs of circadian transcription factors with certain circadian phase relationships can give rise to these 12 hr rhythms, if binding noncompetitively to the promoters of regulated genes. We took an inventory of circadian transcription factors in mouse liver using available microarray data and matched these to predicted binding sites in the promoters of genes displaying 12 hr rhythms. Binding sites for transcription factor pairs with phase relationships predicted by theory were overrepresented in these promoters.
除了动物行为、细胞生物学和基因表达中众所周知的昼夜节律外，还有 12 小时节律。产生这些 12 小时节律的机制尚不清楚。我们的工作假设是，观察到的基因表达 12 小时节律是昼夜节律钟成分相互作用的结果。对小鼠肝脏表达数据的分析确实揭示了观察到的 12 小时基因表达节律中存在强烈的昼夜节律成分。我们从理论上表明，如果非竞争性地结合到受调控基因的启动子上，具有特定昼夜相位关系的成对昼夜转录因子可以产生这些 12 小时节律。我们使用可用的微阵列数据对小鼠肝脏中的昼夜转录因子进行了清查，并将这些因子与显示 12 小时节律的基因启动子中的预测结合位点进行了匹配。具有理论预测相位关系的转录因子对的结合位点在这些启动子中过度表达。
