Grothe C, Unsicker K
Department of Anatomy and Cell Biology, University of Marburg, Federal Republic of Germany.
Histochemistry. 1990;94(2):141-7. doi: 10.1007/BF02440180.
We studied the spatial and temporal pattern of basic fibroblast growth factor (bFGF) immunoreactivity in the rat adrenal gland during postnatal development. In the cortex the glomerulosa zone reveals a strong anti-bFGF immunoreactivity at all developmental ages studied. In the fasciculata zone the high number of anti-bFGF immunoreactive cells in the first week decreases during the second and third week. The late developing reticularis zone shows only few anti-bFGF labeled cells at all postnatal ages. This distributional pattern of bFGF immunoreactivity matches that of mitotic activity in the rat adrenal cortex strengthening the role of bFGF as an autocrine growth factor for adrenocortical cells. In the medulla anti-bFGF positive chromaffin cells become detectable at postnatal day (P) 8 and increase in number during the second and third week. In the adult rat the staining intensity of the chromaffin cells was higher than at P18. In the adult medulla bFGF colocalizes with noradrenaline suggesting its presence in a chromaffin cell subpopulation. In accordance with previous results the role of the chromaffin cell bFGF as a neurotrophic factor for preganglionic sympathetic neurons is discussed.
我们研究了出生后发育过程中大鼠肾上腺中碱性成纤维细胞生长因子（bFGF）免疫反应性的时空模式。在皮质中，球状带在所有研究的发育阶段均显示出强烈的抗bFGF免疫反应性。束状带中，第一周抗bFGF免疫反应性细胞数量较多，在第二和第三周减少。发育较晚的网状带在所有出生后年龄段仅显示少量抗bFGF标记的细胞。bFGF免疫反应性的这种分布模式与大鼠肾上腺皮质有丝分裂活性的模式相匹配，强化了bFGF作为肾上腺皮质细胞自分泌生长因子的作用。在髓质中，抗bFGF阳性嗜铬细胞在出生后第8天（P8）可检测到，在第二和第三周数量增加。在成年大鼠中，嗜铬细胞的染色强度高于P18时。在成年髓质中，bFGF与去甲肾上腺素共定位，表明其存在于嗜铬细胞亚群中。根据先前的结果，讨论了嗜铬细胞bFGF作为节前交感神经元神经营养因子的作用。
