Apte S S
Nuffield Department of Orthopaedic Surgery, University of Oxford, Nuffield Orthopaedic Centre, Headington, UK.
Histochemistry. 1990;94(2):201-4. doi: 10.1007/BF02440188.
The Ki-67 monoclonal antibody which recognizes a human nuclear antigen expressed by cycling cells but not by resting cells was found to react immunohistochemically with tissues from the rabbit Oryctolagus cuniculus. Ki-67 immunoreactivity was restricted to the nucleus. A comparative study with bromodeoxyuridine labelling patterns was carried out to study the association with proliferating cells. In lingual, jejunal and appendix mucosa, skin, adrenal gland, thymus, spleen, bone marrow, testis, growth cartilage, periosteum and periochondrium of long bones the distribution of Ki-67 positive and bromodeoxyuridine labelled cells was similar and consistent with the distribution of proliferating cells in these tissues. In tissue from the brain, kidney, skeletal or cardiac muscle and liver no Ki-67 positive or bromodeoxyuridine labelled cells were seen. In cartilage labelled in vivo with tritiated thymidine, all thymidine labelled cells were also Ki-67 positive. These results suggest that the Ki-67 antibody recognizes a nuclear antigen in the rabbit that is associated with cell proliferation and is expressed by cells in S-phase as well as in other phases of the cell cycle.
Ki-67单克隆抗体可识别由处于增殖周期的细胞而非静止细胞表达的一种人类核抗原，研究发现它能与家兔（穴兔）的组织发生免疫组织化学反应。Ki-67免疫反应性仅限于细胞核。开展了一项与溴脱氧尿苷标记模式的对比研究，以探讨其与增殖细胞的关联。在舌、空肠和阑尾黏膜、皮肤、肾上腺、胸腺、脾脏、骨髓、睾丸、生长软骨、长骨的骨膜和软骨膜中，Ki-67阳性细胞和溴脱氧尿苷标记细胞的分布相似，且与这些组织中增殖细胞的分布一致。在脑、肾、骨骼肌或心肌以及肝脏的组织中，未观察到Ki-67阳性或溴脱氧尿苷标记的细胞。在用氚标记胸腺嘧啶核苷进行体内标记的软骨中，所有胸腺嘧啶核苷标记的细胞也呈Ki-67阳性。这些结果表明，Ki-67抗体识别家兔体内一种与细胞增殖相关的核抗原，该抗原由处于S期以及细胞周期其他阶段的细胞表达。
