大鼠心脏中三磷酸腺苷的区室化：一项31P自旋晶格弛豫研究。

Adenosine triphosphate compartmentation in the rat heart: a 31P spin-lattice relaxation study.

作者信息

Suzuki E, Maeda M, Kuki S, Steward M C, Takami H, Seo Y, Murakami M, Watari H

机构信息

Department of Molecular Physiology, National Institute for Physiological Sciences, Aichi.

出版信息

J Biochem. 1990 Apr;107(4):559-62. doi: 10.1093/oxfordjournals.jbchem.a123086.

DOI:10.1093/oxfordjournals.jbchem.a123086

PMID:2358429

Abstract

Longitudinal relaxation times (T1) of phosphorus compounds in the perfused rat heart and erythrocytes were measured using the 31P Driven-Equilibrium Single-Pulse Observation of T1 relaxation (DESPOT) method at 33 degrees C. Both creatine phosphate in the heart and the three phosphate groups of adenosine triphosphate (ATP) in erythrocytes showed single-exponential relaxation. The three phosphate groups of ATP in the heart, however, had two T1 components. The T1 values of the short and the long T1 components of the beta-phosphate of ATP were ca. 0.4 and 14 s, respectively. The fraction with the long T1 represented ca. 30% of the total ATP content. These results suggested that there were two major pools of intracellular ATP in the rat heart which could be determined by 31P NMR spectroscopy.

摘要

在33摄氏度下，使用31P T1弛豫驱动平衡单脉冲观测（DESPOT）方法测量了灌注大鼠心脏和红细胞中磷化合物的纵向弛豫时间（T1）。心脏中的磷酸肌酸和红细胞中三磷酸腺苷（ATP）的三个磷酸基团均表现出单指数弛豫。然而，心脏中ATP的三个磷酸基团有两个T1成分。ATP的β-磷酸基团的短T1成分和长T1成分的T1值分别约为0.4秒和14秒。长T1成分的比例约占总ATP含量的30%。这些结果表明，大鼠心脏中存在两个主要的细胞内ATP池，可通过31P核磁共振波谱法确定。