Department of Biochemistry and Biophysics, Center for RNA Biology, University of Rochester School of Medicine and Dentistry, Rochester, New York, USA.
Nat Chem Biol. 2013 Jun;9(6):353-5. doi: 10.1038/nchembio.1231. Epub 2013 Apr 14.
PreQ1 riboswitches regulate genes by binding the pyrrolopyrimidine intermediate preQ1 during the biosynthesis of the essential tRNA base queuosine. We report what is to our knowledge the first preQ1-II riboswitch structure at 2.3-Å resolution, which uses a previously uncharacterized fold to achieve effector recognition at the confluence of a three-way helical junction flanking a pseudoknotted ribosome-binding site. The results account for translational control mediated by the preQ1-II riboswitch class and expand the known repertoire of ligand-binding modes used by regulatory RNAs.
PreQ1 类核酶通过在必需 tRNA 碱基 queuosine 的生物合成过程中结合吡咯并嘧啶嘧啶中间产物 PreQ1 来调节基因。我们报告了迄今为止第一个分辨率为 2.3Å 的 PreQ1-II 类核酶结构，该结构使用以前未表征的折叠方式在侧翼三螺旋结的汇合处实现效应物识别，该三螺旋结环绕假结核糖体结合位点。该结果解释了 PreQ1-II 类核酶介导的翻译控制，并扩展了调控 RNA 所使用的已知配体结合模式。
