Dideriksen Kasper, Sindby Ann Kathrine Ryberg, Krogsgaard Michael, Schjerling Peter, Holm Lars, Langberg Henning
Institute of Sports Medicine, Department of Orthopedic Surgery M81, Bispebjerg Hospital, Bispebjerg Bakke 23, DK-2400 Copenhagen, NV, Denmark ; Center for Healthy Aging, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3, DK-2200 Copenhagen, N, Denmark.
Springerplus. 2013 Mar 13;2(1):109. doi: 10.1186/2193-1801-2-109. Print 2013 Dec.
Evidence suggests that habitual loading can result in patellar tendon hypertrophy, especially at the proximal and distal parts of the patellar tendon. The underlying protein kinetic changes and its regulation remains controversial and human data, investigating this topic, are limited. The present study investigated how acute exercise affects growth factor production and collagen fractional synthetic rate in patellar tendon tissue from patients undergoing an anterior cruciate ligament reconstruction operation. The operation was performed by use of the bone-patellar tendon-bone method under spinal anesthesia. Twelve subjects were randomized to one of two groups: a control group or an exercise group (1-hr unilateral knee-extension 67% of Wmax 24 hours before operation). Two hours before the anterior cruciate ligament operation a flooding-dose of L-[1-(13)C]proline was given. Tissue from the most proximal part of the patellar tendon was obtained during the operation. Tendon collagen fractional synthetic rate and mRNA concentrations of TGF-β-1, CTGF, and type I and III collagen were measured. CTGF and type I collagen expression were higher in the exercise group compared to the control group (p < 0.05). Type III collagen expression (p = 0.11), TGF-β-1 expression (p = 0.34), and collagen fractional synthetic rate (p = 0.26) did not differ between groups. Although the expression of CTGF and type I collagen were higher, the patellar tendon collagen fractional synthetic rate was not correspondingly higher after exercise. The elevated CTGF expression in the exercise group indicates that the TGF-beta pathway could be an important link between mechanical loading and stimulation of tendon tissue type I collagen expression.
有证据表明，习惯性负荷可导致髌腱肥大，尤其是在髌腱的近端和远端。潜在的蛋白质动力学变化及其调节仍存在争议，针对这一主题的人体数据有限。本研究调查了急性运动如何影响接受前交叉韧带重建手术患者的髌腱组织中生长因子的产生和胶原蛋白的合成率。手术采用脊髓麻醉下的骨-髌腱-骨方法进行。12名受试者被随机分为两组之一：对照组或运动组（术前24小时进行1小时单侧膝关节伸展，负荷为最大负荷的67%）。在前交叉韧带手术前两小时，给予大剂量的L-[1-(13)C]脯氨酸。手术期间获取髌腱最近端部分的组织。测量肌腱胶原蛋白合成率以及转化生长因子-β-1（TGF-β-1）、结缔组织生长因子（CTGF）、I型和III型胶原蛋白的mRNA浓度。与对照组相比，运动组中CTGF和I型胶原蛋白的表达更高（p < 0.05）。两组之间III型胶原蛋白表达（p = 0.11）、TGF-β-1表达（p = 0.34）和胶原蛋白合成率（p = 0.26）没有差异。尽管运动组中CTGF和I型胶原蛋白的表达较高，但运动后髌腱胶原蛋白合成率并没有相应提高。运动组中CTGF表达升高表明，TGF-β途径可能是机械负荷与肌腱组织I型胶原蛋白表达刺激之间的重要联系。
