Department of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation-State Research Institute, Pulawy, Poland.
J Agric Food Chem. 2013 May 8;61(18):4417-23. doi: 10.1021/jf400153c. Epub 2013 Apr 26.
For the first time Trifolium scabrum L. was researched for its phenolic profile. Sixteen phenolics (isoflavones and flavonoids) were isolated and identified in the aerial parts of T. scabrum L. Their structures were established by electrospray ionization-mass spectrometry (ESI-MS) and nuclear magnetic resonance (NMR) techniques. Quantitative analysis of individual phenolics performed by means of ultraperformance liquid chromatography-mass spectrometry (UPLC-MS) was based on calibration curves obtained for previously isolated standard compounds. Additionally, the free radical scavenging potential of these substances was assessed by means of a simple benchtop thin-layer chromatography-2,2-diphenyl-1-picrylhydrazyl radical (TLC-DPPH(•)) bioassay. Thus, T. scabrum L. can be regarded as a potential source of estrogenic and antioxidant compounds, both of significance in the pharmaceutical as well as the food industry. The results show that T. scabrum L. can be considered as a natural and very good commercial source of phenolic compounds (mainly isoflavones).
首次对三叶草（Trifolium scabrum L.）进行了酚类物质的研究。从三叶草（Trifolium scabrum L.）的地上部分分离并鉴定出 16 种酚类物质（异黄酮和类黄酮）。通过电喷雾电离-质谱（ESI-MS）和核磁共振（NMR）技术确定了它们的结构。通过超高效液相色谱-质谱（UPLC-MS）对各个酚类物质进行定量分析，其基础是先前分离得到的标准化合物的校准曲线。此外，通过简单的台式薄层色谱-2,2-二苯基-1-苦基肼自由基（TLC-DPPH（•））生物测定法评估了这些物质的自由基清除能力。因此，三叶草（Trifolium scabrum L.）可以被视为雌激素和抗氧化化合物的潜在来源，这些化合物在制药和食品工业中都具有重要意义。研究结果表明，三叶草（Trifolium scabrum L.）可以被认为是酚类化合物（主要是异黄酮）的天然且非常好的商业来源。
