Shi Shu Yun, Zhang Yu Ping, Zhou Hong Hao, Huang Ke Long, Jiang Xin Yu
School of Chemistry and Chemical Engineering, Central South University, Changsha, P. R. China.
J Immunoassay Immunochem. 2010;31(3):233-49. doi: 10.1080/10739149.2010.488616.
An online rapid screening method, the high-performance liquid chromatography (HPLC)-diode array detector (DAD)-radical scavenging detection (RSD)-electrospray ionization (ESI)-mass spectroscopy (MS)/MS system, was developed for the screening and identification of radical scavengers from Neo-Taraxacum siphonanthum, a new species found in China in 1989. For further characterization, the target compounds were isolated by silica column chromatography, preparative high-performance liquid chromatography (HPLC), HSCCC, and Sephadex LH-20 column chromatography and elucidated on the basis of ultraviolet (UV), ESI-MS/MS, and nuclear magnetic resonance (NMR) spectroscopy, as well as the chemical analysis. Eighteen antioxidative polyphenols (5 caffeic acid derivatives and 13 flavonoid derivatives) were characterized from Neo-T. siphonanthum. The distribution of all compounds was discussed in a chemosystematic context, which suggested that the genera Neo-Taraxacum and Taraxacum might relate chemosystematically.
开发了一种在线快速筛选方法，即高效液相色谱（HPLC）-二极管阵列检测器（DAD）-自由基清除检测（RSD）-电喷雾电离（ESI）-质谱（MS）/ MS系统，用于从1989年在中国发现的新物种新苦苣菜中筛选和鉴定自由基清除剂。为了进一步表征，通过硅胶柱色谱、制备型高效液相色谱（HPLC）、高速逆流色谱（HSCCC）和葡聚糖LH-20柱色谱分离目标化合物，并基于紫外（UV）、ESI-MS/MS和核磁共振（NMR）光谱以及化学分析对其进行鉴定。从新苦苣菜中鉴定出18种抗氧化多酚（5种咖啡酸衍生物和13种黄酮类衍生物）。在化学分类学背景下讨论了所有化合物的分布，这表明新苦苣菜属和苦苣菜属可能在化学分类学上相关。
