甜椒脂溶性部位的抗癌潜力及 Capsianosides 的鉴定
Anticancer Potential and Capsianosides Identification in Lipophilic Fraction of Sweet Pepper ( L.).
机构信息
Department of Chemistry, Faculty of Food Science and Biotechnology, University of Life Sciences in Lublin, Akademicka 15, 20-950 Lublin, Poland.
Department of Molecular Biotechnology and Genetics, Faculty of Biology and Environmental Protection, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland.
出版信息
Molecules. 2020 Jul 7;25(13):3097. doi: 10.3390/molecules25133097.
This study aimed to determine the health-promoting properties of sweet pepper by comparing the activity of fractions with variable lipophilicity. Fractions from red pericarp: aqueous (F1), 40% MeOH (F2), and 70% MeOH (F3) were analyzed for antiradical activity (with DPPH and ABTS), and the contents of total phenolic compounds (TP), flavonoids (TF), and dihydroxycinnamic acids (TDHCA). The anticancer potential of the fractions was evaluated in vitro using different cancer cell lines: human colorectal carcinoma (HCT116) and PC-3 (prostate cancer cell). Fibroblast-like cells of L929 obtained from subcutaneous adipose tissue of mouse were used as normal cells. The highest content of TP, TF, and TDHCA along with the strongest antiradical activity was observed for fraction F2, while the strongest anticancer properties against PC-3 were observed in fraction F3. Fraction F3 primarily contained capsianoside derivatives, which had been isolated through chromatographic methods and identified by spectral methods. These analyses helped in identifying 8 compounds, including 3 new compounds.
本研究旨在通过比较具有不同亲脂性的馏分的活性，来确定甜椒的促进健康特性。对红色果皮的馏分：水相（F1）、40%甲醇（F2）和 70%甲醇（F3）进行了抗氧化活性（DPPH 和 ABTS）分析，以及总酚化合物（TP）、类黄酮（TF）和二羟基肉桂酸（TDHCA）的含量分析。使用不同的癌细胞系：人结肠直肠癌细胞（HCT116）和前列腺癌细胞（PC-3），在体外评估了馏分的抗癌潜力。从小鼠皮下脂肪组织中获得的成纤维样细胞 L929 被用作正常细胞。F2 馏分表现出最高的 TP、TF 和 TDHCA 含量以及最强的抗氧化活性，而 F3 馏分对 PC-3 的抗癌活性最强。F3 馏分主要含有通过色谱方法分离并通过光谱方法鉴定的 Capsianoside 衍生物。通过这些分析，鉴定出了 8 种化合物，其中包括 3 种新化合物。
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