El-Deftar M F, El Gerzawi S M, Abdel-Azim A A, Tohamy S M
The Department of Pathology, National Cancer Institute, Cairo University, Egypt.
J Egypt Natl Canc Inst. 2012 Mar;24(1):7-14. doi: 10.1016/j.jnci.2011.12.001. Epub 2012 Jan 10.
Despite improvements in diagnosis and therapy of oral and oro-pharyngeal carcinomas during the past 30years the 5-year disease-free survival is still poor. Patient's prognosis is affected by cervical lymph node metastasis rather than primary tumors. The DNA ploidy and S-phase fraction (SPF) are associated with tumor aggressiveness and patient outcome in many solid tumors.
Analysis of DNA ploidy and SPF in primary oral squamous cell carcinoma (OSCC) and corresponding node metastasis as prognostic markers in relation to conventional prognostic factors and disease-free survival (DFS).
Ploidy status and SPF (mean value) of 37 formalin-fixed paraffin embedded (FFPE) primary OSCC tumors and their corresponding lymph node metastasis were assessed by flow cytometry (FCM) and correlated with clinicopathologic prognostic parameters and DFS.
Most of OSCC tumors (86.5%) were Grade II. Among primary OSCC the incidence of aneuploidy was 19%, 51.4% showed high SPF (>10.62%) and 48.6% had low SPF (<10.62%). Border line significance (P=0.10) was detected between ploidy status and SPF in primary tumors. In lymph node metastases all tumors were diploid, 78.4% of metastatic tumors revealed low SPF and only 21.6% showed high SPF. There was a statistically significant correlation (p=0.02) between site of tumors and DFS and a highly statistically significant correlation (p=0.01) between SPF of primary tumors and DFS.
High SPF of primary OSCC tumors assessed by FCM was significantly associated with decreased disease free survival rates. DNA ploidy showed no relationship to bad prognostic indicators in either primary OSCC or their metastatic tumors.
尽管在过去30年里口腔和口咽癌的诊断和治疗有所改善，但5年无病生存率仍然很低。患者的预后受颈部淋巴结转移的影响，而非原发肿瘤。在许多实体瘤中，DNA倍体和S期分数（SPF）与肿瘤侵袭性和患者预后相关。
分析原发性口腔鳞状细胞癌（OSCC）及其相应淋巴结转移中的DNA倍体和SPF，作为与传统预后因素和无病生存率（DFS）相关的预后标志物。
通过流式细胞术（FCM）评估37例福尔马林固定石蜡包埋（FFPE）原发性OSCC肿瘤及其相应淋巴结转移的倍体状态和SPF（平均值），并与临床病理预后参数和DFS进行相关性分析。
大多数OSCC肿瘤（86.5%）为Ⅱ级。在原发性OSCC中，非整倍体的发生率为19%，51.4%显示高SPF（>10.62%），48.6%显示低SPF（<10.62%）。在原发性肿瘤中，倍体状态和SPF之间检测到临界显著性（P=0.10）。在淋巴结转移中，所有肿瘤均为二倍体，78.4%的转移瘤显示低SPF，仅21.6%显示高SPF。肿瘤部位与DFS之间存在统计学显著相关性（p=0.02），原发性肿瘤的SPF与DFS之间存在高度统计学显著相关性（p=0.01）。
通过FCM评估的原发性OSCC肿瘤的高SPF与无病生存率降低显著相关。DNA倍体在原发性OSCC或其转移瘤中均与不良预后指标无关。
