Baretton G, Li X, Stoll C, Fischer-Brandies E, Schmidt M, Löhrs U
Institute of Pathology, Universities of Munich, Germany.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1995 Jan;79(1):68-76. doi: 10.1016/s1079-2104(05)80077-0.
Deoxyribonucleic acid ploidy was determined in paraffin-embedded tumor tissue from 116 patients with primary oral squamous cell carcinomas (including 5 carcinomas of the lip and 14 of the tongue) by means of flow cytometry. One hundred six cases were suitable for evaluation (91%). Sixty-eight percent of the cases (n = 72) showed a nondiploid nuclear DNA content. Nondiploidy correlated significantly with presence of lymph node metastases (p < 0.02) but not with tumor stage, grading (World Health Organization), or relapse-free and overall survival. Carcinomas of the lip and tongue turned out to be diploid more frequently than other oral squamous cell carcinomas (p = 0.002). In the 21 cases in which a comparison of DNA content of excisional biopsy specimens and subsequent resection specimens was possible a difference in DNA ploidy was found in one case only. The comparison of primary tumors and their lymph node metastases in 30 cases revealed a discrepancy of DNA content in five cases (17%), which was connected with a shift from nondiploidy to diploidy in four out of five cases. Fifty cases studied in parallel by means of image cytometry with Feulgen-stained tissue sections exhibited a concordance of the ploidy status in 87% and a significant correlation of the DNA index values obtained with both methods (p < 0.01). These results demonstrate that DNA ploidy in oral squamous cell carcinomas is distributed rather homogeneously within the tumors and remains rather stable in the lymph node metastases. Despite a significant correlation between nondiploidy and presence of lymph node metastases, ploidy failed to be a statistically significant parameter for prognosis in oral squamous cell carcinomas in our investigation.
采用流式细胞术对116例原发性口腔鳞状细胞癌（包括5例唇癌和14例舌癌）石蜡包埋肿瘤组织中的脱氧核糖核酸倍性进行了测定。106例适合评估（91%）。68%的病例（n = 72）显示核DNA含量为非二倍体。非二倍体与淋巴结转移的存在显著相关（p < 0.02），但与肿瘤分期、分级（世界卫生组织）或无复发生存率和总生存率无关。唇癌和舌癌比其他口腔鳞状细胞癌更频繁地表现为二倍体（p = 0.002）。在21例能够比较切除活检标本和后续切除标本DNA含量的病例中，仅在1例中发现了DNA倍性差异。对30例原发性肿瘤及其淋巴结转移的比较显示，5例（17%）存在DNA含量差异，其中5例中有4例与从非二倍体向二倍体的转变有关。通过对Feulgen染色组织切片进行图像细胞术平行研究的50例病例中，倍性状态的一致性为87%，两种方法获得的DNA指数值具有显著相关性（p < 0.01）。这些结果表明，口腔鳞状细胞癌中的DNA倍性在肿瘤内分布相当均匀，在淋巴结转移中保持相当稳定。尽管非二倍体与淋巴结转移的存在之间存在显著相关性，但在我们的研究中，倍性未能成为口腔鳞状细胞癌预后的统计学显著参数。
