Laboratory of Hepatobiliary and Liver Transplantation, Department of Surgery, University Hospital of Zurich, Zurich, Switzerland.
J Surg Res. 2013 Oct;184(2):e1-7. doi: 10.1016/j.jss.2013.03.022. Epub 2013 Mar 30.
Mouse models of liver transplantation are powerful tools for biomedical research. The cuff method is currently the most popular approach for revascularization of mouse liver grafts, as it is relatively easy to perform hence reducing the anhepatic time. However, the use of cuffs may induce a tissue reaction, causing chronic obstruction of anastomosed vessels, leading to portal hypertension. Here, we applied the suture technique for arterialized liver transplantation in mice.
Liver transplantation was performed on 14 pairs of C57BL/6 mice. All hepatic vessels were anastomosed by sewing. The bile duct was connected with a stent. The liver grafts were harvested for histology on day 30 after surgery. Serum aspartate transaminase, alkaline phosphatase and bilirubin were measured at d 3, 7, and 30 after implantation.
With a mean anhepatic time of 25.78 ± 3 min, the survival rate was 86% (n = 14) at 30 d following surgery. During this period, no significant liver injury was observed as assessed by serum markers and histology. Survival remained stable when grafts were exposed to 6 h cold ischemia prior to implantation. Vessel examination at the end of the studied period revealed an intact patency and a lack of collateral vessel growth.
Arterialized liver transplantation with sewed revascularization in mice is technically feasible. Both sewing and arterialization seem to be important factors promoting the survival of mouse recipients. The mouse model of suture arterialized orthotopic liver transplantation provides a novel tool for modern transplantation research and might be particularly suited for studies requiring longer-term survival of recipients.
肝移植的小鼠模型是生物医学研究的有力工具。套管法是目前用于鼠肝移植物再血管化的最常用方法，因为它相对容易操作，从而减少无肝时间。然而，套管的使用可能会引起组织反应，导致吻合血管的慢性阻塞，从而导致门脉高压。在这里，我们应用缝合技术进行动脉化肝移植的小鼠模型。
在 14 对 C57BL/6 小鼠进行肝移植。所有肝血管均通过缝合吻合。胆管与支架相连。术后第 30 天取肝移植物进行组织学检查。植入后第 3、7 和 30 天测量血清天冬氨酸转氨酶、碱性磷酸酶和胆红素。
平均无肝时间为 25.78±3 分钟，术后 30 天的存活率为 86%（n=14）。在此期间，通过血清标志物和组织学检查未观察到明显的肝损伤。在植入前将移植物暴露于 6 小时冷缺血时，存活率保持稳定。研究结束时的血管检查显示通畅性完好，无侧支血管生长。
在小鼠中进行缝合血管重建的动脉化肝移植在技术上是可行的。缝合和动脉化似乎都是促进小鼠受体存活的重要因素。缝合动脉化原位肝移植的小鼠模型为现代移植研究提供了一种新工具，可能特别适合需要受体长期存活的研究。
