Pan Ning, Liu Zhenzhen, He Jinjing, Li Song, Lv Xiangwei, Wang Liming, Liu Qinlong
Department of Anesthesiology, The Second Affiliated Hospital of Dalian Medical University, Dalian, Liaoning Province, China.
Department of General Surgery, The Second Affiliated Hospital of Dalian Medical University, Dalian, Liaoning Province, China; Dalian Medical University, Dalian, Liaoning Province, China.
PLoS One. 2015 Jul 24;10(7):e0133030. doi: 10.1371/journal.pone.0133030. eCollection 2015.
The mouse model of arterialized orthotopic liver transplantation (AOLT) has played an important role in biomedical research. The available methods of sutured anastomosis for reconstruction of the hepatic artery are complicated, resulting in a high incidence of complications and failure. Therefore, we developed and evaluated a new model of AOLT in mice.
Male inbred C57BL/6 mice were used in this study. A continuous suture approach was applied to connect the suprahepatic inferior vena cava (SHVC). The portal vein and infrahepatic inferior vena cava (IHVC) were connected according to the "two-cuff" method. The common bile duct was connected by a biliary stent. We used the stent (G3 group) or aortic trunk (G2 group) to reconstruct the hepatic artery. The patency of the hepatic artery was verified by transecting the artery near the graft after one week. The survival rate of the recipients and serum alanine aminotransferase (ALT) levels, hepatic pathologic alterations, apoptosis and necrosis were observed at one week postoperatively.
The patency of the hepatic artery was verified in eight of ten mice in G3 and in six of ten mice in G2. The 7-day survival rate, extents of necrosis and apoptosis, and TGF-β levels were not significantly different among the three groups (P>0.05). However, the serum ALT levels and operation time were markedly lower in G3 compared with G2 or G1 (both P<0.05).
Reconstruction of the hepatic artery using a stent can be performed quickly with a high rate of patency. This model simplifies hepatic artery anastomosis and should be promoted in the field of biomedical research.
动脉化原位肝移植（AOLT）小鼠模型在生物医学研究中发挥了重要作用。现有的用于重建肝动脉的缝合吻合方法复杂，导致并发症和失败的发生率较高。因此，我们开发并评估了一种新的小鼠AOLT模型。
本研究使用雄性近交系C57BL/6小鼠。采用连续缝合方法连接肝上下腔静脉（SHVC）。门静脉和肝下下腔静脉（IHVC）根据“双袖套”方法进行连接。胆总管通过胆管支架进行连接。我们使用支架（G3组）或主动脉干（G2组）重建肝动脉。一周后，通过在移植物附近横断动脉来验证肝动脉的通畅情况。术后一周观察受体的存活率、血清丙氨酸氨基转移酶（ALT）水平、肝脏病理改变、细胞凋亡和坏死情况。
G3组10只小鼠中有8只、G2组10只小鼠中有6只肝动脉通畅得到验证。三组之间的7天存活率、坏死和凋亡程度以及TGF-β水平无显著差异（P>0.05）。然而，与G2组或G1组相比，G3组的血清ALT水平和手术时间明显更低（均P<0.05）。
使用支架重建肝动脉可以快速完成且通畅率高。该模型简化了肝动脉吻合，应在生物医学研究领域推广。
