Oldani Graziano, Lacotte Stéphanie, Orci Lorenzo A, Delaune Vaihere, Slits Florence, Gex Quentin, Morel Philippe, Rubbia-Brandt Laura, Toso Christian
Division of Abdominal Surgery, Department of Surgery, Faculty of Medicine, University of Geneva, Geneva, Switzerland.
Hepato-Pancreato-Biliary Centre, Geneva University Hospitals, Geneva, Switzerland.
Liver Transpl. 2016 Dec;22(12):1688-1696. doi: 10.1002/lt.24637.
Because of the wide availability of genetically modified animals, mouse orthotopic liver transplantation is often preferred over rat liver transplantation. We present a simplified mouse liver transplantation technique and compare transplantation outcomes with versus without hepatic artery anastomosis. Instruments for liver implantation were designed and printed with a 3-dimensional (3D) printer. The suprahepatic vena cava anastomosis was performed with a 10-0 running suture. The vena porta and infrahepatic vena cava were joined on extraluminal cuffs, using the 3D-printed device for spatial alignment and stabilization. The hepatic artery was reconstructed in half of the recipients using intraluminal stents. Liver function tests (3, 7, and 28 days) and histology (7 and 28 days) were assessed after transplantation. We performed 22 consecutive syngeneic C57BL/6 mouse orthotopic liver transplantations. The median portal clamping time was 12.5 ± 1.5 minutes. The survival rate at 4 weeks was 100% for both arterialized and nonarterialized recipients (n = 7, 4 recipients of each group being killed for early histology at day 7). Liver function tests at 3, 7, and 28 days were similar between arterialized versus nonarterialized groups. Liver parenchyma demonstrated only irrelevant abnormalities in both groups. The proposed device allows for a shorter clamping time compared with the published literature. Using this technique, the artery does not need to be anastomosed, with no impact on graft and recipient outcomes. The device is available for 3D printing. Liver Transplantation 22 1688-1696 2016 AASLD.
由于基因改造动物易于获得，小鼠原位肝移植通常比大鼠肝移植更受青睐。我们展示了一种简化的小鼠肝移植技术，并比较了有或没有肝动脉吻合的移植结果。用于肝植入的器械是用三维（3D）打印机设计并打印的。肝上下腔静脉吻合采用10-0连续缝合。门静脉和肝下腔静脉在管外袖套上连接，使用3D打印装置进行空间对齐和固定。一半的受体使用腔内支架重建肝动脉。移植后评估肝功能测试（第3、7和28天）和组织学（第7和28天）。我们连续进行了22例同基因C57BL/6小鼠原位肝移植。门静脉阻断的中位时间为12.5±1.5分钟。动脉化和非动脉化受体在4周时的生存率均为100%（n = 7，每组4只受体在第7天处死用于早期组织学检查）。动脉化组和非动脉化组在第3、7和28天的肝功能测试结果相似。两组肝实质均仅显示无关紧要的异常。与已发表的文献相比，所提出的装置可缩短阻断时间。使用该技术，无需吻合动脉，对移植物和受体的结果没有影响。该装置可供3D打印。《肝脏移植》22 1688 - 1696 2016美国肝病研究学会
