Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247 667, India.
Biosens Bioelectron. 2013 Sep 15;47:307-12. doi: 10.1016/j.bios.2013.03.025. Epub 2013 Mar 21.
A multi-walled carbon nano tube (MWCNT) modified pyrolytic graphite (MPG) electrode is prepared and applied to detect norfloxacin (NFX) based on its electrochemical reduction. The experimental parameters affecting the NFX determination were optimized in terms of MWCNT amount, pH, reaction time, and square wave frequency. The dynamic range for the NFX analysis ranged between 1.2 and 1000µM with a detection limit of 40.6±3.3nM. The effect of NFX on the catabolism of caffeine has been studied by determining its concentration in the urine samples after the prolonged administration of NFX using the MPG electrode. The results show that the catabolism of caffeine is inhibited by ~65% after five days of NFX administration, consequently the caffeine concentration in the urine sample is increased, which is reflected in terms of ~2.5 times increase in the peak current of caffeine. The determinations of NFX and caffeine were selective and the method was successfully applied in biological fluids and pharmaceutical tablets for the test compound analysis. In future this method can be useful for the selective determination of NFX and studying its effect on caffeine catabolism.
一种多壁碳纳米管（MWCNT）修饰的热解石墨（MPG）电极被制备出来，并应用于基于电化学还原的诺氟沙星（NFX）检测。根据 MWCNT 用量、pH 值、反应时间和方波频率等实验参数对 NFX 测定的影响进行了优化。NFX 分析的动态范围在 1.2 到 1000µM 之间，检测限为 40.6±3.3nM。通过使用 MPG 电极在长时间给予 NFX 后测定尿液样品中的 NFX 浓度，研究了 NFX 对咖啡因代谢的影响。结果表明，NFX 给药 5 天后，咖啡因的代谢被抑制约 65%，因此尿液中咖啡因的浓度增加，这反映在咖啡因的峰电流增加了约 2.5 倍。NFX 和咖啡因的测定具有选择性，该方法已成功应用于生物体液和药用片剂中的测试化合物分析。在未来，该方法可用于 NFX 的选择性测定以及研究其对咖啡因代谢的影响。
