Theanponkrang Somjai, Suginta Wipa, Weingart Helge, Winterhalter Mathias, Schulte Albert
School of Chemistry, Institute of Science, Nakhon Ratchasima, Thailand ; Biochemistry- Electrochemistry Research Unit, Institute of Science, Nakhon Ratchasima, Thailand.
Biochemistry- Electrochemistry Research Unit, Institute of Science, Nakhon Ratchasima, Thailand ; School of Biochemistry, Suranaree University of Technology, Nakhon Ratchasima, Thailand.
Int J Nanomedicine. 2015 Jan 27;10:859-68. doi: 10.2147/IJN.S75237. eCollection 2015.
A new automated pharmacoanalytical technique for convenient quantification of redox-active antibiotics has been established by combining the benefits of a carbon nanotube (CNT) sensor modification with electrocatalytic activity for analyte detection with the merits of a robotic electrochemical device that is capable of sequential nonmanual sample measurements in 24-well microtiter plates. Norfloxacin (NFX) and ciprofloxacin (CFX), two standard fluoroquinolone antibiotics, were used in automated calibration measurements by differential pulse voltammetry (DPV) and accomplished were linear ranges of 1-10 μM and 2-100 μM for NFX and CFX, respectively. The lowest detectable levels were estimated to be 0.3±0.1 μM (n=7) for NFX and 1.6±0.1 μM (n=7) for CFX. In standard solutions or tablet samples of known content, both analytes could be quantified with the robotic DPV microtiter plate assay, with recoveries within ±4% of 100%. And recoveries were as good when NFX was evaluated in human serum samples with added NFX. The use of simple instrumentation, convenience in execution, and high effectiveness in analyte quantitation suggest the merger between automated microtiter plate voltammetry and CNT-supported electrochemical drug detection as a novel methodology for antibiotic testing in pharmaceutical and clinical research and quality control laboratories.
通过将碳纳米管（CNT）传感器修饰对分析物检测的电催化活性的优点与能够在24孔微量滴定板中进行连续非手动样品测量的机器人电化学装置的优点相结合，建立了一种用于方便定量氧化还原活性抗生素的新型自动化药物分析技术。诺氟沙星（NFX）和环丙沙星（CFX）这两种标准氟喹诺酮抗生素用于通过差分脉冲伏安法（DPV）进行的自动校准测量，NFX和CFX的线性范围分别为1-10μM和2-100μM。估计NFX的最低可检测水平为0.3±0.1μM（n = 7），CFX为1.6±0.1μM（n = 7）。在已知含量的标准溶液或片剂样品中，两种分析物都可以通过机器人DPV微量滴定板测定法定量，回收率在100%的±4%以内。当在添加了NFX的人血清样品中评估NFX时，回收率也很好。使用简单的仪器、操作方便以及分析物定量的高效性表明，自动微量滴定板伏安法与CNT支持的电化学药物检测相结合是制药和临床研究以及质量控制实验室中抗生素检测的一种新方法。
