Department of Pharmaceutical Sciences, COMSATS Institute of Information Technology, Abbottabad, Pakistan.
Analyst. 2013 Jun 7;138(11):3104-16. doi: 10.1039/c3an00031a.
Miniaturized bioanalytical systems are increasingly being used in the field of biochemical research. Immobilized enzyme microbioreactors in capillary electrophoresis (CE) have been constructed and used to fulfill the increasing demands for miniaturized bioanalytical systems. This manipulation permits low sample consumption, reduced costs, short analysis times and efficient analyses. This review provides an overview of the distinct characteristics of immobilized microbioreactors in CE. After an introduction on miniaturized microreactors, the various methods of enzyme immobilization will be discussed. The emphasis will be on two common constructions of microreactors in CE, i.e. CE-coupled microreactors, and microreactors directly integrated into the CE capillary. Such microbioreactors offer straightforward automation of reaction steps followed by separation in the same capillary.
微型化生物分析系统在生化研究领域得到了越来越广泛的应用。固定化酶微生物反应器在毛细管电泳（CE）中得到了构建和应用，以满足对微型化生物分析系统的日益增长的需求。这种操作允许低样品消耗、降低成本、缩短分析时间和高效分析。本综述概述了固定化微生物反应器在 CE 中的独特特点。在介绍微型化微反应器之后，将讨论各种酶固定化方法。重点将放在 CE 中两种常见的微反应器结构上，即 CE 耦合微反应器和直接集成到 CE 毛细管中的微反应器。这种微生物反应器提供了在同一毛细管中进行反应步骤的自动化，然后进行分离。
