Directional screening and identification of potential cytotoxic components from by a combination of surface palsmon resonance and chromatography.

OBJECTIVE

To establish a method for directional screening of the cytotoxic components from the medicinal herb of by a combination of surface plasmon resonance (SPR) biosensor and chromatographic isolation technology.

METHODS

Under the guidance of bioactive assessment based on binding abilities between objects and the α-Mannosidase (α-Man) target, the active components from different solvents extracts, different polar extraction parts and fractions were screened orderly and directionally using SPR. Components with a high binding ability to α-Man can be precisely oriented in a narrower fractions range and are easy to isolate. Three human cancer cells were used to evaluate the cytotoxic activity of component with the highest affinity to α-Man.

RESULTS

Eight compounds were isolated and identificated from for the first time. Deoxyvasicinone possessed the highest affinity to α-Man among them. Moreover, deoxyvasicinone showed good effects on inhibited proliferation of human hepatoma cells HepG2 (IC = 5.7 μmol/L), human breast cancer cells MCF7 (IC = 7.21 μmol/L) and human lung cancer cells HCC827 (IC = 0.75 μmol/L), respectively. In particular, its inhibitory effect on HCC827 was stronger than the positive drug gefitinib (IC = 1.65 μmol/L).

CONCLUSION

A comprehensive strategy of directional screening potential cytotoxic components from herb based on biomolecular interaction and chromatography was established. Deoxyvasicinone as an effective anti-cancer component was initially isolated from . It is expected that this screening strategy could provide new perspectives for rapid screening and identification of active components from natural plants with the complex matrix.