State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing, China.
PLoS One. 2013 Apr 12;8(4):e60547. doi: 10.1371/journal.pone.0060547. Print 2013.
DNA methylation controls many aspects of plant growth and development. Here, we report a novel annual growth potential change that may correlate with changes in levels of the major DNA demethylases and methyltransferases in cotton ovules harvested at different times of the year. The abundances of DNA demethylases, at both the mRNA and protein levels, increased significantly from February to August and decreased during the remainder of the 12-month period, with the opposite pattern observed for DNA methyltransferases. Over the course of one year, substantial changes in methylcytosine content was observed at certain CHH sites (H = A, C, or T) in the promoter regions of the ETHYLENE RESPONSIVE FACTOR 6 (ERF6), SUPPRESSION OF RVS 161 DELTA 4 (SUR4) and 3-KETOACYL-COA SYNTHASE 13 (KCS13), which regulate cotton fiber growth. Three independent techniques were used to confirm the annual fluctuations in DNA methylation. Furthermore, in homozygous RNAi lines specifically targeting REPRESSOR OF SILENCING 1 (ROS1, a conserved DNA demethylase domain), promotion of DNA methylation significantly reduced fiber growth during August.
DNA 甲基化控制着植物生长和发育的许多方面。在这里，我们报道了一种新的年度生长潜力变化，这种变化可能与棉花胚珠中主要去甲基酶和甲基转移酶水平的变化有关，这些胚珠是在一年中不同时间收获的。从 2 月到 8 月，DNA 去甲基酶的 mRNA 和蛋白质水平的丰度显著增加，而在 12 个月的剩余时间内则减少，而 DNA 甲基转移酶则呈现相反的模式。在一年的时间里，在 ETHYLENE RESPONSIVE FACTOR 6（ERF6）、SUPPRESSION OF RVS 161 DELTA 4（SUR4）和 3-KETOACYL-COA SYNTHASE 13（KCS13）的启动子区域中，某些 CHH 位点（H=A、C 或 T）的甲基胞嘧啶含量发生了显著变化，这些基因调控棉花纤维的生长。使用三种独立的技术来证实 DNA 甲基化的年度波动。此外，在专门针对沉默抑制物 1（ROS1，一种保守的 DNA 去甲基酶结构域）的纯合 RNAi 系中，促进 DNA 甲基化在 8 月份显著降低了纤维的生长。
