Department of Neurosurgery, Albert Einstein College of Medicine and Montefiore Medical Center, Bronx, New York, USA.
PLoS One. 2013 Apr 12;8(4):e61752. doi: 10.1371/journal.pone.0061752. Print 2013.
The growing body of clinical and experimental data regarding electromagnetic field (EMF) bioeffects and their therapeutic applications has contributed to a better understanding of the underlying mechanisms of action. This study reports that two EMF modalities currently in clinical use, a pulse-modulated radiofrequency (PRF) signal, and a static magnetic field (SMF), applied independently, increased the rate of deoxygenation of human hemoglobin (Hb) in a cell-free assay. Deoxygenation of Hb was initiated using the reducing agent dithiothreitol (DTT) in an assay that allowed the time for deoxygenation to be controlled (from several min to several hours) by adjusting the relative concentrations of DTT and Hb. The time course of Hb deoxygenation was observed using visible light spectroscopy. Exposure for 10-30 min to either PRF or SMF increased the rate of deoxygenation occurring several min to several hours after the end of EMF exposure. The sensitivity and biochemical simplicity of the assay developed here suggest a new research tool that may help to further the understanding of basic biophysical EMF transduction mechanisms. If the results of this study were to be shown to occur at the cellular and tissue level, EMF-enhanced oxygen availability would be one of the mechanisms by which clinically relevant EMF-mediated enhancement of growth and repair processes could occur.
关于电磁场 (EMF) 生物效应及其治疗应用的越来越多的临床和实验数据有助于更好地了解其作用的潜在机制。本研究报告称，目前临床使用的两种 EMF 模式，即脉冲调制射频 (PRF) 信号和静磁场 (SMF)，单独应用时，可增加细胞外血红蛋白 (Hb) 脱氧速率的测定。在脱氧剂二硫苏糖醇 (DTT) 的测定中，起始 Hb 的脱氧作用，该测定允许通过调整 DTT 和 Hb 的相对浓度来控制脱氧时间 (从几分钟到几个小时)。使用可见光光谱观察 Hb 脱氧的时间过程。暴露于 PRF 或 SMF 10-30 分钟可增加 EMF 暴露结束后几分钟至数小时内发生的脱氧速率。这里开发的测定方法的灵敏度和生化简单性表明，这是一种新的研究工具，可能有助于进一步了解基本的生物物理 EMF 转导机制。如果本研究的结果表明在细胞和组织水平上发生，那么 EMF 增强的氧气供应将是临床相关的 EMF 介导的生长和修复过程增强发生的机制之一。
