Kusuyama T, Fujita M, Okuyama Y, Taguchi T
Department of Surgery, Research Institute for Microbial Diseases, Osaka University, Japan.
Nihon Geka Gakkai Zasshi. 1990 Mar;91(3):303-12.
The subrenal capsule assay for cancer chemotherapy was performed, using tumor-specimens of 19 patients' cancers. Twelve tumor-specimens were implanted simultaneously under the renal space of immunocompetent CDF1 mice, cyclosporin A (CsA) 60 mg/kg treated mice, and BALB/c-nu/nu (nude) mice. The persistence and growth of implanted tumor-xenografts of each mouse, was evaluated, on day 6 and 9 after inoculation. The tumor-xenografts implanted under the renal space of immunocompetent mice, grew larger on days 6 in 9 cases, but histological evaluation showed tumor tissues were in various degree replaced by host reactive tissues. Host reaction in CsA-treated mice or nude mice was suppressed almost completely, but the persistence and proliferation of tumor-xenografts of both mice was varied, depending on the nature of original tumors. The judgment for cancer chemotherapy on our modified SRC assay was almost similar between CsA-treated mice and nude mice, but there were some cases in which macroscopical judgment didn't correspond with histological effect. The DNA synthesis of tumor-xenografts of 7 patients, was examined by using sequential changes of BrdU labeling index (LI) in the renal space of CsA-treated mice. It was showed LI rather indicated the nature of original tumors itself.
采用19例癌症患者的肿瘤标本进行了癌症化疗的肾包膜下测定。将12个肿瘤标本同时植入免疫活性CDF1小鼠、环孢素A（CsA）60mg/kg处理的小鼠和BALB/c-nu/nu（裸）小鼠的肾间隙。在接种后第6天和第9天评估每只小鼠植入的肿瘤异种移植物的持久性和生长情况。植入免疫活性小鼠肾间隙的肿瘤异种移植物，在第6天有9例生长更大，但组织学评估显示肿瘤组织不同程度地被宿主反应性组织取代。CsA处理的小鼠或裸鼠中的宿主反应几乎完全受到抑制，但两种小鼠肿瘤异种移植物的持久性和增殖情况各不相同，这取决于原发肿瘤的性质。在我们改良的SRC测定中，对癌症化疗的判断在CsA处理的小鼠和裸鼠之间几乎相似，但在一些情况下，宏观判断与组织学效果不一致。利用CsA处理的小鼠肾间隙中BrdU标记指数（LI）的连续变化，检测了7例患者肿瘤异种移植物的DNA合成。结果表明，LI更能反映原发肿瘤本身的性质。
