Faculty of Chemistry, Materials and Bioengineering, Department of Chemistry and Materials Engineering, Kansai University, 3-3-35 Yamate-cho, Suita-shi, Osaka, 564-8680, Japan.
J Biomater Sci Polym Ed. 2013;24(7):882-95. doi: 10.1080/09205063.2012.710823. Epub 2012 Aug 8.
This work reports the effect of polyphosphoester ionomers on mineralization and cell viability in comparison with the fundamental actions of bisphosphonates. Poly(2-benzoxy-2-oxo-1,3,2-dioxaphospholane-co-2-ethoxy-2-oxo-1,3,2-dioxaphospholane) was synthesized by ring-opening polymerization using an organocatalyst. The benzyl group of polyphosphoesters was completely deprotected as a result of preparing polyphosphoester ionomers. The sodium salt of the ionomer was also prepared by neutralization with an aqueous NaOH solution. The amount of polyphosphoester ionomers adsorbed on hydroxyapatite (HAp) microparticles increased with an increase in the number of ionized units in the polyphosphoesters. The affinity of the ionomers for the HAp microparticles was enhanced by the formation of sodium salt. The adsorption mass of the ionomers having 0.09 ionized unit fraction was approximately equal to that of pamidronate when in contact with HAp microparticles from their 24 μM solution. Compared to pamidronate, polyphosphoester ionomers could significantly reduce HAp formation. Furthermore, similar to that of pamidronate, the resorption of HAp was also reduced with the addition of polyphosphoester ionomers. The cytotoxicity of polyphosphoester ionomers was significantly lower than that of pamidronate and the cell compatibility of the polyphosphoester ionomer was further improved by sodium salt formation. Selective adsorption of polyphosphoester ionomers on the mineral deposits generated from MC3T3-E1 cells was also observed.
本工作报道了多聚磷酸酯离子聚合物对矿化和细胞活力的影响，与双膦酸盐的基本作用进行了比较。通过使用有机催化剂的开环聚合合成了聚（2-苯氧-2-氧代-1,3,2-二氧杂磷杂环戊烷-co-2-乙氧基-2-氧代-1,3,2-二氧杂磷杂环戊烷）。多聚磷酸酯的苄基基团由于制备多聚磷酸酯离子聚合物而完全脱保护。通过用含水 NaOH 溶液中和来制备离子聚合物的钠盐。多聚磷酸酯离子聚合物在羟基磷灰石（HAp）微粒上的吸附量随着多聚磷酸酯中离子化单元数量的增加而增加。离子聚合物对 HAp 微粒的亲和力通过形成钠盐而增强。当与 HAp 微粒接触时，具有 0.09 离子化单元分数的离子聚合物的吸附质量约等于帕米膦酸盐的吸附质量，而当接触 24μM 溶液中的 HAp 微粒时，其吸附质量约等于帕米膦酸盐的吸附质量。与帕米膦酸盐相比，多聚磷酸酯离子聚合物可以显著减少 HAp 的形成。此外，与帕米膦酸盐类似，多聚磷酸酯离子聚合物的添加也减少了 HAp 的再吸收。多聚磷酸酯离子聚合物的细胞毒性明显低于帕米膦酸盐，通过形成钠盐进一步提高了多聚磷酸酯离子聚合物的细胞相容性。还观察到多聚磷酸酯离子聚合物对 MC3T3-E1 细胞产生的矿物质沉积物的选择性吸附。
