Cheng Yanyan, Xue Jiangdong, Yao Chunyu, Gao Li, Ma Dexing, Liu Yan, Zhang Zhigang
College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
J Vet Med Sci. 2013;75(9):1139-46. doi: 10.1292/jvms.13-0004. Epub 2013 Apr 16.
The objective of this study was to evaluate the possible protection of resveratrol against lung injury induced by arsenic trioxide (As2O3). Twenty-four healthy Chinese Dragon Li cats of either sex were randomly divided into four groups: control (1 ml/kg physiological saline), As2O3 (1 mg/kg), resveratrol (3 mg/kg) and resveratrol (3 mg/kg) + As2O3 (1 mg/kg). The resveratrol + As2O3- treated group was given resveratrol 1 hr before As2O3 (1 mg/kg) administration. We found that pretreatment with resveratrol in a clinically comparable dose regimen can reversed the changes in morphological and biochemical parameters induced by As2O3 in the lung. Resveratrol treatment also upregulated the activities of antioxidant enzymes and attenuated As2O3-induced increases in reactive oxygen species, 8-hydroxydeoxyguanosine and malondialdehyde production in the lung. In addition, resveratrol attenuated the As2O3-induced reduction in the ratio of reduced glutathione to oxidized glutathione, the content of total glutathione and lung arsenic burden. These findings indicated that resveratrol can provide significant protection against As2O3-induced oxidative damage.
本研究的目的是评估白藜芦醇对三氧化二砷（As2O3）诱导的肺损伤的可能保护作用。将24只健康的中华狸花猫（雌雄不限）随机分为四组：对照组（1 ml/kg生理盐水）、As2O3组（1 mg/kg）、白藜芦醇组（3 mg/kg）和白藜芦醇（3 mg/kg）+As2O3（1 mg/kg）组。白藜芦醇+As2O3处理组在给予As2O3（1 mg/kg）前1小时给予白藜芦醇。我们发现，以临床可比剂量方案用白藜芦醇预处理可逆转As2O3诱导的肺形态和生化参数变化。白藜芦醇治疗还上调了抗氧化酶的活性，并减轻了As2O3诱导的肺中活性氧、8-羟基脱氧鸟苷和丙二醛生成的增加。此外，白藜芦醇减轻了As2O3诱导的还原型谷胱甘肽与氧化型谷胱甘肽比值、总谷胱甘肽含量和肺砷负荷的降低。这些发现表明，白藜芦醇可对As2O3诱导的氧化损伤提供显著保护。
