眼球摘除术对大鼠视交叉上核和上丘中血管活性肠肽免疫反应性神经元的相反作用。

Contrary effect of eye enucleation on VIP-immunoreactive neurons in the suprachiasmatic nucleus and the superior colliculus of the rat.

作者信息

Okamoto S, Okamura H, Takahashi Y, Akagi Y, Yanaihara N, Ibata Y

机构信息

Department of Ophthalmology, Kyoto Prefectural University of Medicine, Japan.

出版信息

Neurosci Lett. 1990 May 4;112(2-3):137-42. doi: 10.1016/0304-3940(90)90192-c.

DOI:10.1016/0304-3940(90)90192-c

PMID:2359513

Abstract

The topographic alternation of vasoactive intestinal peptide (VIP) immunoreactivity after long-term bilateral eye enucleation (80 days) was investigated in the suprachiasmatic nucleus (SCN) and the superior colliculus of the adult rat with the peroxidase-antiperoxidase method. In the SCN, the immunostaining of VIP-immunoreactive (IR) cell bodies increased, and the intensity and density of VIP-IR fibers and terminals were markedly enhanced after eye enucleation. On the other hand, after eye enucleation, no VIP-IR cell bodies and fibers were visible in the stratum griseum superficiale of the superior colliculus, although fusiform-shaped VIP-IR neurons were distributed in the layer of unoperated control rats. The present results, therefore, have revealed that the long-term blockage of retinal input induces changes of VIP immunoreactivity in different manners, according to the target brain areas.

摘要

采用过氧化物酶-抗过氧化物酶法，研究成年大鼠长期双侧眼球摘除（80天）后，视交叉上核（SCN）和上丘中血管活性肠肽（VIP）免疫反应性的地形学变化。在SCN中，VIP免疫反应性（IR）细胞体的免疫染色增加，眼球摘除后VIP-IR纤维和终末的强度和密度明显增强。另一方面，眼球摘除后，上丘浅灰质层中未见VIP-IR细胞体和纤维，尽管在未手术的对照大鼠层中有梭形VIP-IR神经元分布。因此，目前的结果表明，视网膜输入的长期阻断会根据目标脑区以不同方式诱导VIP免疫反应性的变化。