Denis P, Dussaillant M, Nordmann J P, Bérod A, Saraux H, Rostène W
INSERM U339, Paris, France.
Graefes Arch Clin Exp Ophthalmol. 1993 Sep;231(9):541-5. doi: 10.1007/BF00921120.
The localization of the messenger RNA (mRNA) encoding vasoactive intestinal peptide/peptide histidine isoleucine (VIP/PHI) in the rat eye was studied by in situ hybridization histochemistry using a synthetic 35S-labeled oligodeoxyribonucleotide. Among the layers of the retina, specific labeling was found in the soma of some cells in the innermost lamina of the inner nuclear layer. Occasionally, labeled cells were also present in the ganglion cell layer. No specific labeling was detected in any ocular structures other than the retina, including the cornea, iris, ciliary body, choroid, choriocapillaris, optic nerve and sclera. We also examined VIP/PHI mRNA expression in the suprachiasmatic nucleus (SCN) of the hypothalamus, which is the pacemaker of circadian rhythms and synchronizes them with the environmental light-dark cycle. High labeling was found in the ventrolateral part of the nucleus, which is the site of most projections from the retina. Monocular enucleation of rats at birth resulted in an increase of VIP/PHI mRNA expression in SCN ipsilateral to the removed eye after postnatal day 60. The discrete pattern of hybridization for VIP/PHI mRNA indicates that these two peptides are synthetized in rat retina and SCN and suggests that they may play a key role in the photic entrainment to the SCN regulating various circadian rhythms.
采用合成的35S标记寡脱氧核糖核苷酸，通过原位杂交组织化学方法研究了大鼠眼中编码血管活性肠肽/肽组氨酸异亮氨酸（VIP/PHI）的信使核糖核酸（mRNA）的定位。在视网膜各层中，在内核层最内层的一些细胞的胞体中发现了特异性标记。偶尔，神经节细胞层中也有标记细胞。除视网膜外，在包括角膜、虹膜、睫状体、脉络膜、脉络膜毛细血管、视神经和巩膜在内的任何眼部结构中均未检测到特异性标记。我们还检查了下丘脑视交叉上核（SCN）中VIP/PHI mRNA的表达，视交叉上核是昼夜节律的起搏器，并使其与环境明暗周期同步。在该核的腹外侧部分发现了高标记，这是来自视网膜的大多数投射的部位。出生时对大鼠进行单眼摘除，导致出生后60天移除眼同侧的SCN中VIP/PHI mRNA表达增加。VIP/PHI mRNA杂交的离散模式表明，这两种肽在大鼠视网膜和SCN中合成，提示它们可能在光信号传入SCN调节各种昼夜节律中起关键作用。
