阿尔茨海默病中的脑膜流动性和脂质过氧化作用。

Brain membrane fluidity and lipid peroxidation in Alzheimer's disease.

作者信息

Hajimohammadreza I, Brammer M

机构信息

Department of Neuroscience, Institute of Psychiatry, London, U.K.

出版信息

Neurosci Lett. 1990 May 4;112(2-3):333-7. doi: 10.1016/0304-3940(90)90226-y.

DOI:10.1016/0304-3940(90)90226-y

PMID:2359533

Abstract

Membrane fluidity and lipid peroxidation in 4 brain areas from patients with Alzheimer's disease (AD) and matched controls were determined by measuring fluorescence anisotropy of the lipophilic probe 1,6-diphenyl-1,3,5-hexatriene (DPH) and by the thiobarbituric acid test respectively. Fluorescence anisotropy of DPH was not changed in any of the 4 areas in AD compared to controls. Basal levels of malondialdehyde (MDA; an intermediate in the lipid peroxidation process) were also not changed in different brain regions of AD and controls. However, stimulated MDA production determined by incubating tissue with FeSO4 plus H2O2 produced significantly higher MDA levels in AD brain than in controls.

摘要

通过测量亲脂性探针1,6 - 二苯基 - 1,3,5 - 己三烯（DPH）的荧光各向异性以及分别采用硫代巴比妥酸试验，测定了阿尔茨海默病（AD）患者和匹配对照者4个脑区的膜流动性和脂质过氧化情况。与对照组相比，AD患者4个脑区中任何一个区域的DPH荧光各向异性均未改变。丙二醛（MDA；脂质过氧化过程中的一种中间体）的基础水平在AD患者和对照组的不同脑区也未改变。然而，通过用硫酸亚铁加过氧化氢孵育组织所测定的刺激后MDA生成量，AD脑区显著高于对照组。