Department of Botany and Plant Pathology, Oregon State University Corvallis, OR, USA ; Center for Genome Research and Biocomputing, Oregon State University Corvallis, OR, USA.
Front Microbiol. 2013 Apr 11;4:83. doi: 10.3389/fmicb.2013.00083. eCollection 2013.
Important progress in understanding replication, interactions with host plants, and evolution of closteroviruses enabled engineering of several vectors for gene expression and virus-induced gene silencing. Due to the broad host range of closteroviruses, these vectors expanded vector applicability to include important woody plants such as citrus and grapevine. Furthermore, large closterovirus genomes offer genetic capacity and stability unrivaled by other plant viral vectors. These features provided immense opportunities for using closterovirus vectors for the functional genomics studies and pathogen control in economically valuable crops. This review briefly summarizes advances in closterovirus research during the last decade, explores the relationships between virus biology and vector design, and outlines the most promising directions for future application of closterovirus vectors.
在理解复制、与宿主植物的相互作用以及杆状病毒进化方面取得的重要进展，使人们能够设计出几种用于基因表达和病毒诱导基因沉默的载体。由于杆状病毒的广泛宿主范围，这些载体扩大了载体的适用性，包括柑橘和葡萄等重要的木本植物。此外，大型杆状病毒基因组提供了其他植物病毒载体无法比拟的遗传容量和稳定性。这些特性为利用杆状病毒载体进行功能基因组学研究和经济价值作物的病原体控制提供了巨大的机会。本文简要总结了过去十年中杆状病毒研究的进展，探讨了病毒生物学与载体设计之间的关系，并概述了未来杆状病毒载体应用最有前景的方向。
