Sierra-Mejia Andrea, Villamor Dan E V, Rocha Aaron, Wintermantel William M, Tzanetakis Ioannis E
Department of Entomology and Plant Pathology, Division of Agriculture, University of Arkansas System Fayetteville, Arkansas 72701, United States.
United States Department of Agriculture, Agricultural Research Service, Salinas, California 93905, United States.
Virus Res. 2024 Dec;350:199488. doi: 10.1016/j.virusres.2024.199488. Epub 2024 Nov 7.
Criniviruses are emerging pathogens responsible for significant disease outbreaks worldwide. Among them, blackberry yellow vein-associated virus (BYVaV) is prevalent in blackberry-producing areas of the United States and, when present in the blackberry yellow vein disease complex with other viruses, can lead to substantial crop losses. To better understand BYVaV biology and its role in virus complex disease development, we developed a BYVaV-derived infectious clone and a virus-induced gene silencing (VIGS) vector. The infectious clone successfully induced systemic infection and symptom development in Nicotiana benthamiana. Additionally, transmission of the recombinant virus to indicator plants was confirmed using the whitefly vector Trialeurodes vaporariorum. The infectious clone was subsequently modified into a VIGS vector, with the foreign insert remaining stable for the length of the study. This work provides essential tools for advancing the study of BYVaV biology and conducting genomic studies in its natural hosts.
番茄潜隐病毒属是在全球范围内引发重大疾病爆发的新兴病原体。其中，黑莓黄脉相关病毒（BYVaV）在美国黑莓产区普遍存在，当它与其他病毒共同存在于黑莓黄脉病复合体中时，会导致大量作物损失。为了更好地了解BYVaV生物学特性及其在病毒复合病害发展中的作用，我们构建了一个源自BYVaV的感染性克隆和一个病毒诱导基因沉默（VIGS）载体。该感染性克隆成功地在本氏烟草中诱导了系统感染和症状发展。此外，使用烟粉虱载体温室粉虱证实了重组病毒向指示植物的传播。随后，该感染性克隆被改造成一个VIGS载体，在研究期间外源插入片段保持稳定。这项工作为推进BYVaV生物学研究以及在其自然寄主中进行基因组研究提供了重要工具。
