Zhao Shijing, Sai Na, Ren Dalin, Xu Bei, Liu Chuan, Huang Guowei
Department of Nutrition and Food Hygiene, School of Public Health, Tianjin Medical University, Tianjin 300070, China.
Wei Sheng Yan Jiu. 2013 Jan;42(1):110-3.
To develop a method for determination of S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH) simultaneously in plasma by reversed-phase high performance liquid chromatography (HPLC).
The plasma were hydrolyzed with trichloroacetic acid 400 g/L, then the hydrolysate was adjusted to centrifuged and filtrated with a 0.45 microm membrane. The supernatants were separated on Venusil MP-C18 column (4.6 mm x 250 mm, 5 microm) at 30 degrees C with a mobile phase of 50 mmol/L NaH2PO4 (pH 4.38), C7 H15, NaO3 S and methanol, a flow rate of 1.0 ml/ min, and UV detection at 254nm.
The SAM and SAH in the corresponding concentration range showed a good linear relation with its peak area, correlation coefficient r > 0.9999, recovery was 94.14% - 102.44%, RSD was 1.00% - 8.10%. The content of SAM and SAH in plasma of healthy people was 0.032 - 0.080 mg/L and 0.004 - 0.010 mg/L respectively.
The validated method is simple, rapid accurate and reliable to the determination of SAM and SAH in plasma.
建立一种反相高效液相色谱法同时测定血浆中S-腺苷甲硫氨酸（SAM）和S-腺苷高半胱氨酸（SAH）的方法。
血浆用400 g/L三氯乙酸水解，水解产物经离心后用0.45μm滤膜过滤。上清液在Venusil MP-C18柱（4.6 mm×250 mm，5μm）上于30℃分离，流动相为50 mmol/L NaH2PO4（pH 4.38）、C7H15、NaO3S和甲醇，流速为1.0 ml/min，于254nm处进行紫外检测。
SAM和SAH在相应浓度范围内与其峰面积呈良好的线性关系，相关系数r＞0.9999，回收率为94.14%～102.44%，相对标准偏差为1.00%～8.10%。健康人血浆中SAM和SAH的含量分别为0.032～0.080 mg/L和0.004～0.010 mg/L。
该验证方法用于血浆中SAM和SAH的测定简便、快速、准确、可靠。
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