Castro Rita, Struys Eduard A, Jansen Erwin E W, Blom Henk J, de Almeida Isabel Tavares, Jakobs Cornelis
Department of Clinical Chemistry, Free University Hospital, Amsterdam, The Netherlands.
J Pharm Biomed Anal. 2002 Jul 31;29(5):963-8. doi: 10.1016/s0731-7085(02)00121-8.
A simplified reversed phase HPLC system for the detection of fluorescent 1,N(6)-etheno derivatives of SAM (S-adenosylmethionine) and S-adenosylhomocysteine (SAH) is described. The most important changes from the previously reported method are a shorter derivatization reaction time, the use of a solid-phase extraction resulting in an increase of the method's sensitivity, and the use of only one chromatographic system to separate SAM and SAH (in which the use of an ion-pairing reagent in the mobile phase is avoided). The linearity of the method was established, and the intra-assay coefficients of variation were 10.4 and 4.7% for SAM and SAH, respectively. Normal plasma values (n=8), evaluated with the present methodology, were, for SAM and SAH, respectively, 57+/-12 and 28+/-3 nM (mean+/-SD).
本文描述了一种用于检测S-腺苷甲硫氨酸（SAM）和S-腺苷高半胱氨酸（SAH）的荧光1,N(6)-乙烯基衍生物的简化反相高效液相色谱系统。与先前报道的方法相比，最重要的变化是衍生化反应时间缩短、采用固相萃取提高了方法的灵敏度，以及仅使用一个色谱系统分离SAM和SAH（避免在流动相中使用离子对试剂）。建立了该方法的线性关系，SAM和SAH的批内变异系数分别为10.4%和4.7%。用本方法评估的正常血浆值（n = 8），SAM和SAH分别为57±12和28±3 nM（平均值±标准差）。
