She Q B, Nagao I, Hayakawa T, Tsuge H
United Graduate School of Agricultural Science, Gifu University, Japan.
Biochem Biophys Res Commun. 1994 Dec 30;205(3):1748-54. doi: 10.1006/bbrc.1994.2871.
A simple method using isocratic HPLC with ultraviolet detection was established for the simultaneous measurement of S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH) in rat tissues. The method provides rapid resolution of both compounds in a single run by direct injection of the perchloric acid extract of tissue, so that sampling procedures and analytical errors can be reduced in determining the SAM/SAH ratio, a measure of transmethylation reactions. The assay has a detection limit of 25 pmol and is linear in the range 50-200 pmol. This procedure was applied to determine the change of SAM/SAH ratio in liver caused by vitamin B6 deficiency. The marked reduction of the SAM/SAH ratios in vitamin B6-deficient rats were due to a drastic rise in SAH concentration with a concurrent striking decrease in SAM concentration.
建立了一种采用等度高效液相色谱法结合紫外检测的简单方法，用于同时测定大鼠组织中的S-腺苷甲硫氨酸（SAM）和S-腺苷同型半胱氨酸（SAH）。该方法通过直接注入组织的高氯酸提取物，在一次运行中即可快速分离这两种化合物，从而在测定SAM/SAH比值（一种转甲基反应的指标）时可减少采样程序和分析误差。该测定法的检测限为25皮摩尔，在50 - 200皮摩尔范围内呈线性。此方法用于测定维生素B6缺乏引起的肝脏中SAM/SAH比值的变化。维生素B6缺乏大鼠中SAM/SAH比值的显著降低是由于SAH浓度急剧升高，同时SAM浓度显著下降。
