Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305-5329, USA.
Dev Cell. 2013 Apr 15;25(1):5-13. doi: 10.1016/j.devcel.2013.03.016.
Elucidation of cellular and gene regulatory networks (GRNs) governing organ development will accelerate progress toward tissue replacement. Here, we have compiled reference GRNs underlying pancreas development from data mining that integrates multiple approaches, including mutant analysis, lineage tracing, cell purification, gene expression and enhancer analysis, and biochemical studies of gene regulation. Using established computational tools, we integrated and represented these networks in frameworks that should enhance understanding of the surging output of genomic-scale genetic and epigenetic studies of pancreas development and diseases such as diabetes and pancreatic cancer. We envision similar approaches would be useful for understanding the development of other organs.
阐明调控器官发育的细胞和基因调控网络（GRNs）将加速组织替代的进展。在这里，我们通过整合多种方法（包括突变分析、谱系追踪、细胞纯化、基因表达和增强子分析以及基因调控的生化研究）的数据挖掘，编译了胰腺发育的参考 GRNs。使用既定的计算工具，我们将这些网络整合并表示在框架中，这应该有助于理解基因组规模的遗传和表观遗传研究以及糖尿病和胰腺癌等疾病的胰腺发育的大量输出。我们设想类似的方法对于理解其他器官的发育也将是有用的。
