Geriatric Research Education and Clinical Center, South Texas Veterans Health Care System, San Antonio, TX 78229, USA.
Exp Biol Med (Maywood). 2013 Mar;238(3):276-84. doi: 10.1177/1535370213482442.
Induced pluripotent stem cells (iPS cells) are important for the future development of regenerative medicine involving autologous cell therapy. Before autologous cell therapy can be applied to human patients, suitable animal models must be developed, and in this context non-human primate models are critical. We previously characterized several lines of marmoset iPS cells derived from newborn skin fibroblasts. In the present studies, we explored methods for the directed differentiation of marmoset iPS cells in the neuroectodermal lineage. In this process we used an iterative process in which combinations of small molecules and protein factors were tested for their effects on mRNA levels of genes that are markers for the neuroectodermal lineage. This iterative process identified combinations of chemicals/factors that substantially improved the degree of marker gene expression over the initially tested combinations. This approach should be generally valuable in the directed differentiation of pluripotent cells for experimental cell therapy.
诱导多能干细胞（iPS 细胞）对于涉及自体细胞治疗的再生医学的未来发展非常重要。在自体细胞治疗可以应用于人类患者之前，必须开发合适的动物模型，在这种情况下，非人类灵长类动物模型至关重要。我们之前已经对从新生皮肤成纤维细胞中获得的几种狨猴 iPS 细胞系进行了特征描述。在本研究中，我们探索了定向分化狨猴 iPS 细胞为神经外胚层谱系的方法。在此过程中，我们使用了一种迭代过程，其中测试了小分子和蛋白质因子的组合对标记神经外胚层谱系的基因的 mRNA 水平的影响。这种迭代过程确定了化学物质/因子的组合，这些组合大大提高了标记基因表达的程度，超过了最初测试的组合。这种方法应该在用于实验性细胞治疗的多能细胞的定向分化中具有普遍价值。
