Hovdenes J, Kvien T K, Hovdenes A B
Institute of Immunology and Rheumatology, National Hospital of Norway, Oslo.
Scand J Rheumatol. 1990;19(3):177-82. doi: 10.3109/03009749009095040.
The purpose of this investigation was to measure interleukin 6 (IL-6) levels in synovial fluid (SF) and plasma from patients with rheumatoid arthritis (RA) and other inflammatory arthritides (non-RA) and to examine the in vitro production of IL-6 by T cells and monocytes from SF and peripheral blood (PB). All patients had high levels of IL-6 in SF. Although the median level was higher in the group of non-RA arthritides, the difference was not statistically significant. Supernatants from both unstimulated and stimulated highly purified CD4+ and CD8+ cells from SF and PB did not contain IL-6, while high levels of IL-6 were detectable in supernatants of mononuclear cells (MNC) and plastic adherent cells without any specific in vitro stimulant. Since IL-6 is so readily produced by normal mononuclear cells in vitro without any specific stimulant, spontaneous in vitro production by SF MNC cannot be considered as evidence for in vivo production by the same cells.
本研究的目的是测量类风湿关节炎（RA）患者及其他炎性关节炎（非RA）患者滑液（SF）和血浆中白细胞介素6（IL-6）的水平，并检测SF和外周血（PB）中T细胞和单核细胞体外产生IL-6的情况。所有患者的SF中IL-6水平均较高。虽然非RA关节炎组的中位数水平更高，但差异无统计学意义。来自SF和PB的未刺激及刺激后的高度纯化CD4+和CD8+细胞的上清液中均未检测到IL-6，而在无任何特异性体外刺激物的单核细胞（MNC）和贴壁细胞的上清液中可检测到高水平的IL-6。由于正常单核细胞在体外无需任何特异性刺激物就能轻易产生IL-6，因此不能将SF MNC的体外自发产生视为同一细胞体内产生的证据。
